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MABN2552-25UL Anti-alpha B-crystallin Antibody, clone aB1788G7G6

Anti-alpha B-crystallin, clone aB1788G7G6, Cat. No. MABN2552, is a highly specific mouse monoclonal antibody that targets Alpha-crystallin B chain and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Inhibition Assay, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: CRYAB, CRYA2, HSOB5 UniProt Number: P02511
MABN2552-25UL
25 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MELISA, IF, IHC, Inhibition, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2552-25UL
      DescriptionAnti-alpha B-crystallin Antibody, clone aB1788G7G6
      Alternate Names
      • Alpha-crystallin B chain
      • Alpha(B)-crystallin
      • Heat shock protein beta-5
      • HspB5
      • Renal carcinoma antigen NY-REN-27
      • Rosenthal fiber component
      Background InformationAlpha-crystallin B chain (UniProt: P02511; also known as Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component) is encoded by the CRYAB (also known as CRYA2, HSOB5) gene (Gene ID: 1410) in human. Alpha-crystallin is a small heat shock protein that is composed of A and B subunits that display about 55% sequence homology. They both serve as molecular chaperones. They are generally present in a molar ratio of 3:1 (alphaA:alphaB). AlphaB is a stress-inducible form that is present in lens, retina, heart and skeletal muscle, and kidney. However, alpha, which is non-stress inducible form is present mainly in lens. Higher expression of alphaB has also been reported in several cancers, including gliomas, renal cell carcinoma, and metaplastic breast carcinomas. Phosphorylation of alphaB is shown to reduce its oligomerization and its anti-apoptotic activities. As a negative regulator of inflammation, it is shown to protect against multiple sclerosis and block ischemic injury, brain stroke, and spinal cord contusion injury in animal models. Mutations in CRYAB gene is reported to cause myofibrillar myopathies characterized at ultrastructural level by disintegration of the sarcomeric Z disk and myofibrils. (Ref.: Nahomi, RB., et al. (2019). J. Immunol. Methods. 467; 37-47).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Applications
      ApplicationAnti-alpha B-crystallin, clone aB1788G7G6, Cat. No. MABN2552, is a highly specific mouse monoclonal antibody that targets Alpha-crystallin B chain and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Inhibition Assay, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Inhibition
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected alpha B-crystallin in mouse eye and human retina tissue lysate.

      Immunohistochemistry Analysis: A representative lot detected alpha B-crystallin in Immunohistochemistry applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

      ELISA Analysis: A representative lot detected alpha B-crystallin in ELISA applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

      Inhibition Analysis: A representative lot inhibited the chaperone and anti-apoptotic activities of alpha B-crystallin. (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

      Immunofluorescence Analysis: A representative lot detected alpha B-crystallin in Immunofluorescence applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

      Western Blotting Analysis: A representative lot detected alpha B-crystallin in Western Blotting applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Alpha(B)-crystallin.
      CloneaB1788G7G6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone aB1788G7G6 is a mouse monoclonal antibody that detects Alpha(B)-crystallin. It targets an epitope within 21 amino acids within the N-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine, Monkey, Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CRYAB
      • CRYA2
      • HSOB5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~20 kDa observed; 20.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse retina tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected alpha B-crystallin in mouse retina tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN2552-25UL 04061841369554

      Documentation

      Anti-alpha B-crystallin Antibody, clone aB1788G7G6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-alpha B-crystallin Antibody, clone aB1788G7G6 Certificates of Analysis

      TitleLot Number
      Anti-alpha B-crystallin, clone aB1788G7G6 - Q3380060 Q3380060

      References

      Reference overviewPub Med ID
      A monoclonal antibody targeted to the functional peptide of αB-crystallin inhibits the chaperone and anti-apoptotic activities.
      Nahomi, RB; Nandi, SK; Nagaraj, RH
      J Immunol Methods  467  37-47  2019

      Show Abstract       		30738041 30738041