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ABN2265 Anti-alpha-Synuclein Antibody, oligomer-specific Syn33

Anti-alpha-Synuclein, oligomer-specific Syn33 Antibody, Cat. No. ABN2265, is a highly specific rabbit polyclonal antibody that targets aggregted alpha-synuclein and has been tested in ELISA, Inhibition, Immunofluorescence, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: SCNA, NACP, PARK1 UniProt Number: P37840
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ABN2265
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, Inhibition, IF, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN2265
      DescriptionAnti-alpha-Synuclein Antibody, oligomer-specific Syn33
      Alternate Names
      • oligomeric alpha-synuclein
      • Non-A beta component of AD amyloid
      • Non-A4 component of amyloid precursor
      • NACP
      Background InformationAlpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson's disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. Aggregates of alpha-synuclein are present in Lewy bodies. Alpha-synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. Mutations in the SNCA gene produce defective alpha-synuclein that induces conformational changes making it more prone to self-aggregation and deposition in Lewy bodies. Mutant forms of alpha-synuclein impair synaptic vesicle formation, elevate cytoplasmic levels of dopamine, and increases superoxide radicals, which lead to oxidative stress and misfolding of alpha-synuclein.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS.
      Quality LevelMQ100
      Applications
      ApplicationAnti-alpha-Synuclein, oligomer-specific Syn33 Antibody, Cat. No. ABN2265, is a highly specific rabbit polyclonal antibody that targets aggregted alpha-synuclein and has been tested in ELISA, Inhibition, Immunofluorescence, and Western Blotting.
      Key Applications
      • ELISA
      • Inhibition
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected alpha-Synuclein, oligomers in brain cortices from Parkinson s disease (PD) and dementia with Lewy bodies (DLB) patients (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).

      Western Blotting Analysis: A representative lot detected recombinant alpha-Synuclein, oligomers. (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).

      Western Blotting Analysis: A representative lot detected recombinant alpha-Synuclein, oligomer. (Courtesy of Dr. Rakez Kayed's laboratory at University of Texas Medical Branch, Galveston).

      ELISA Analysis: A representative lot detected alpha-Synuclein, oligomers in ELISA application (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).

      Inhibition Analysis: A representative lot inhibited cytotoxicity exerted alpha-synuclein oligomers in human neuroblastoma SH-SY5Y cells measured by MTT-based assay (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
      Biological Information
      ImmunogenSynuclein oligomers from full length human wild-type alpha-synuclein.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically recognizes aggregated form of alpha-synuclein.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SCNA
      • NACP
      • PARK1
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~75 kDa observed; 14.46 kDa calculated (monomers). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Alpha Synuclein aggregate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected alpha-Synuclein in alpha Synuclein aggregates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABN2265 04054839362880

      Documentation

      Anti-alpha-Synuclein Antibody, oligomer-specific Syn33 Certificates of Analysis

      TitleLot Number
      Anti-alpha-Synuclein, oligomer-specific Syn33 - 3172417 3172417
      Anti-alpha-Synuclein, oligomer-specific Syn33 - 3449080 3449080
      Anti-alpha-Synuclein, oligomer-specific Syn33 - 3481390 3481390
      Anti-alpha-Synuclein, oligomer-specific Syn33 - 3731418 3731418
      Anti-alpha-Synuclein, oligomer-specific Syn33 - 3905506 3905506
      Anti-alpha-Synuclein, oligomer-specific Syn33 - 4077597 4077597
      Anti-alpha-Synuclein, oligomer-specific Syn33 Polyclonal Antibody Q2933437
      Anti-alpha-Synuclein, oligomer-specific Syn33 Polyclonal Antibody 3102326