Anti-csBAP31, clone SP1-B7

Clone SP1-B7 is a mouse monoclonal antibody that specifically detects cell surface B-cell receptor-associated protein 31 in human.

Anti-csBAP31, clone SP1-B7, Cat. No. MABC1712, is a mouse monoclonal antibody that detects cell surface B-cell receptor-associated protein 31 (csBAP31) and is tested for use in Flow Cytometry, Immunoprecipitation, and Western Blotting.

Flow Cytometry Immunoprecipitation Western Blotting

Flow Cytometry Analysis: A representative lot detected csBAP31 in Flow Cytometry applications (Seo, S.R., et. al. (2017). PLoS One. 12(11):e0188075).

Immunoprecipitation Analysis: A representative immunoprecipitated csBAP31 in Immunoprecipitation applications (Seo, S.R., et. al. (2017). PLoS One. 12(11):e0188075).

Western Blotting Analysis: A representative lot detected csBAP31 in Western Blotting applications (Seo, S.R., et. al. (2017). PLoS One. 12(11):e0188075).

A549 human carcinoma sphere cells.

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

B-cell receptor-associated protein 31 (UniProt: P51572; also known as BCR-associated protein 31, BAP31, 6C6-AG tumor-associated antigen, Protein CDM, p28) is encoded by the BCAP31 (also known as BAP31, DXS1357E) gene (Gene ID: 10134) in human. BAP31 is an endoplasmic reticulum (ER) membrane protein that is also expressed on surface (csBAP31) of embryonic stem cells. ER associated BAP1 palsy a significant role as a molecular chaperone for the newly synthesized transmembrane proteins and also acts as an important regulator of apoptosis. It interacts with pro-caspase-8L and Bcl-2/Bcl-xL on the ER membrane and is cleaved by caspase-8. The resulting cleaved product, p20, is an important inducer of apoptosis. The cell surface expressed BAP31 is shown to promote cell survival through the regulation of cell adhesion to the extracellular matrix in human embryonic stem cells. csBAP31 is also suggested to be a putative pro-apoptotic flag on cancer cells. Its expression is shown to be enhanced in caspase3/7 high A549 cells and csBAP31-positive cells display diminished survival capacity. Higher levels of csBAP31 are reported to contribute to poor survival cancer cells. Clone SP1-B7 specifically recognizes the cell surface-expressed BAP31. (Ref.: Seo, SR et al. (2017). PLoS ONE 12(11): e0188075).

Evaluated by Western Blotting in A549 cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected csBAP31 in A549 cell lysates.

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