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MABF2672-25UL Anti-fHbp Antibody, clone JAR 1

Anti-fHbp, clone JAR 1, Cat. No. MABF2672, is a mouse monoclonal antibody that detects Meningococcal Factor H-binding protein (fHbp) and is tested for use in Dot Blot, ELISA, Inhibition Assay, and Western Blotting.

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MABF2672-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2672-25UL
      DescriptionAnti-fHbp Antibody, clone JAR 1
      Alternate Names
      • Factor H-binding protein
      • Lipoprotein
      • Lipoprotein GNA1870
      Background InformationFactor H-binding protein (UniProt: Q6QCC2; also known as Lipoprotein, Lipoprotein GNA1870) is encoded by the gna1870 (also known as fhbp) gene (Gene ID: 2827909) in Neisseria meningitidis. Neisseria meningitidis, an encapsulated Gram-negative bacterium, is an important cause of bacterial sepsis and meningitis. FHbp, a surface exposed lipoprotein, is present in all strains of Neisseria meningitidis but is usually sparsely expressed. It is reported to be the major receptor for human Factor H (FH), a down-regulatory protein of the alternate complement pathway. Recruitment of FH is the major contributory factor to the ability of this bacteria to avoid innate immune response via inhibition of complement-mediated lysis. High affinity between human FH and FHbp is reported to be responsible for sequestering FH in the plasma and down-regulation of the complement system, which in the absence of down-regulation can cause complement-mediated damage to the bacteria. FHbp exists in two major antigenic sub-families known as A and B that do display minimal cross-protective activity. FHbp also has been divided into three variant groups based on its amino acid sequence. Variant group 1 belongs to subfamily B and accounts for about 60% of disease-causing serogroup B isolates. Subfamily A includes variant groups 2 and 3. Antibodies generated against variant 1 are reported to be bactericidal only against bacterial strains expressing FHbp in the variant 1 group but not against strains that express variants 2 and 3 and vice versa. Clone JAR1 is shown to react with an epitope expressed only by ET5 complex strain MC58. It is shown to confer protection against strain NZ98/254. (Ref.: Vu, DM et al., (2012). Sci. Rep. 2: 341; Beernink, PT et al., (2009). Mol. Immunol. 46(8-9); 1647-1653; Welsch, JA., et al. (2004). J. Immunol. 172(9); 5606-5615).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG3 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-fHbp, clone JAR 1, Cat. No. MABF2672, is a mouse monoclonal antibody that detects Meningococcal Factor H-binding protein (fHbp) and is tested for use in Dot Blot, ELISA, Inhibition Assay, and Western Blotting.
      Key Applications
      • Dot Blot
      • Inhibition
      • Western Blotting
      • ELISA
      Application NotesInhibition Assay: A representative lot of this antibody inhibited binding of factor H to factor H binding protein (fHbp).(Giuntini, S., et al. (2012). PLoS One. 7(3):e34272).

      Western Blotting Analysis: A representative lot detected fHbp in Western Blotting applications (Welsch, J.A., et al. (2004). J Immunol. 172(9):5606-15).

      ELISA Analysis: A representative lot detected fHbp in ELISA applications (Welsch, J.A., et al. (2004). J Immunol. 172(9):5606-15; Giuntini, S., et al. (2012). PLoS One. 7(3):e34272; Vu, D.M., et al. (2012). Sci Rep. 2:341).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full -length recombinant Factor H-binding protein (fHbp) from Neisseria meningitidis.
      EpitopeC-terminal half
      CloneJAR1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone JAR1 is a mouse monoclonal antibody that detects Factor H binding protein (fHbp) from Neisseria meningitidis. It targets an epitope within the C-terminal half.
      IsotypeIgG3
      Species Reactivity
      • Bacteria
      Species Reactivity NoteNeisseria meningitidis
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • gna1870
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight28.99 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot analysis with recombinant fHbp.

      DOT Blot Analysis: Various concentrations of recombinant rfHbp 1D1 (variant 1); rfHbp 1D77 (variant 2); and rfHbp 1D28 (variant 3) were probed with Anti-fHbp, clone JAR1 at 1:250 dilution. This antibody specifically detected rfHbp 1D1 (variant 1).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF2672-25UL 04065267613376