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05-1592 Anti-mTOR Antibody, clone 21D8.2

Anti-mTOR Antibody, clone 21D8.2 is an antibody against mTOR for use in WB & IC.

Anti-mTOR Antibody, clone 21D8.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: FLJ44809, FRAP, FRAP1, FRAP2, OTTHUMP00000001983, RAFT1, RAPT1, mTOR UniProt Number: P42345
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05-1592
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1592
      Replaces04-385
      DescriptionAnti-mTOR Antibody, clone 21D8.2
      Alternate Names
      • FK506 binding protein 12-rapamycin associated protein 1
      • FK506 binding protein 12-rapamycin associated protein 2
      • FK506-binding protein 12-rapamycin complex-associated protein 1
      • FKBP-rapamycin associated protein
      • FKBP12-rapamycin complex-associated protein 1
      • Mammalian target of rapamycin
      • Rapamycin target protein
      • mechanistic target of rapamycin (serine/threonine kinase)
      • rapamycin and FKBP12 target 1
      • rapamycin associated protein FRAP2
      Background InformationmTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Hek293 cell lysate
      PresentationPurified immunoglobulin containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl and 0.05% Sodium Azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-mTOR Antibody, clone 21D8.2 is an antibody against mTOR for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution of this antibody from a previous lot detected mTOR in C2C12, HeLa, and NIH/3T3 cell lysates.
      Biological Information
      ImmunogenFlag-tagged full-length mTOR protein
      Clone2ID8.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes mTOR.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteProven to react with human and mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
      Gene Symbol
      • FLJ44809
      • FRAP
      • FRAP1
      • FRAP2
      • OTTHUMP00000001983
      • RAFT1
      • RAPT1
      • mTOR
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTIION: Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
      CATALYTIC ACTIVITY ATP + a protein = ADP + a phosphoprotein.
      ENZYME REGULATION: Kinase activity is positively regulated by RHEB and negatively regulated by DEPDC6.
      SUBUNIT STRUCTURE: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains FRAP1, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains FRAP1, MLST8, PROTOR1, RICTOR and MAPKAP1. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PROTOR1 and RICTOR within the mTORC2 complex. Interacts with UBQLN1. Interacts with DEPDC6.
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side.
      TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
      PTM: Autophosphorylated; when part of mTORC1 or mTORC2.
      SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 7 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
      SEQUENCE CAUTION: The sequence AAC39933.1 differs from that shown. Reason: Frameshift at positions 956 and 999.
      Molecular Weight289 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.
      Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      05-1592 04053252586217

      Documentation

      Anti-mTOR Antibody, clone 21D8.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-mTOR Antibody, clone 21D8.2 Certificates of Analysis

      TitleLot Number
      Anti-mTOR, clone 21D8.2 - 3182611 3182611
      Anti-mTOR, clone 21D8.2 - 3204352 3204352
      Anti-mTOR, clone 21D8.2 - 3379935 3379935
      Anti-mTOR, clone 21D8.2 - 3597290 3597290
      Anti-mTOR, clone 21D8.2 - 3854590 3854590
      Anti-mTOR, clone 21D8.2 - 3904347 3904347
      Anti-mTOR, clone 21D8.2 - 4199614 4199614
      Anti-mTOR, clone 2ID8.2 - 2119002 2119002
      Anti-mTOR, clone 2ID8.2 - 2462850 2462850
      Anti-mTOR, clone 2ID8.2 - 2031348 2031348
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies