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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP09 Sigma-AldrichAnti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801)

This Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IP, Paraffin Sections for the detection of p53 (Ab-2) (Pantropic).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Recommended Products

Overview

Replacement Information

Key Specifications Table

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

Pricing & Availability

Catalog Number	Availability	Packaging	Qty/Pack	Price	Quantity
OP09-100UG	Retrieving availability... — Limited Availability Stocked Discontinued Limited Quantities Available Available Login to See Inventory Stocked Limited Availability Stocked Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic ampoule	100 μg	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
OP09-200UG	Retrieving availability... — Limited Availability Stocked Discontinued Limited Quantities Available Available Login to See Inventory Stocked Limited Availability Stocked Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic ampoule	200 μg	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites

Description
Overview	Recognizes the ~53 kDa wild-type and mutant p53 protein in A-431 cells and breast carcinoma tissue.
Catalogue Number	OP09
Brand Family	Calbiochem®

References
References	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative control	SK-OV-3 cells or normal skin
Positive control	A431 cells or breast carcinoma tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Original Clone Legros, Y., et al. 1994. Oncogene 9, 2071. Epitope Identification Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.
Key Applications	Frozen Sections Gel Shift Immunoblotting (Western Blotting) Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (5 µg/ml) Gel Shift (see comments) Immunoblotting (2.5 µg/ml) Immunoprecipitation (1 µg/sample) Paraffin Sections (5 µg/ml, pepsin, trypsin, or heat pre-treatment required)
Application Comments	Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with ³⁵S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using Cat. No. PC35 and chemiluminescent detection. For a gel shift assay, use Cat. No. OP09L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a human p53 fusion protein
Immunogen	Human
Epitope	within amino acids 46-55 of human p53
Clone	PAb1801
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
OP09-100UG	04055977225013
OP09-200UG	04055977225020

Documentation

Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) SDS

Title
Safety Data Sheet (SDS)

Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) Certificates of Analysis

Title	Lot Number
OP09	Enter Lot Number

References

Reference overview
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-October-2007 RFH
Application	Frozen Sections (5 µg/ml) Gel Shift (see comments) Immunoblotting (2.5 µg/ml) Immunoprecipitation (1 µg/sample) Paraffin Sections (5 µg/ml, pepsin, trypsin, or heat pre-treatment required)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
Background	The human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
Host	Mouse
Immunogen species	Human
Immunogen	a human p53 fusion protein
Epitope	within amino acids 46-55 of human p53
Clone	PAb1801
Isotype	IgG₁
Species	human, not mouse, not rat
Positive control	A431 cells or breast carcinoma tissue
Negative control	SK-OV-3 cells or normal skin
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with ³⁵S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using Cat. No. PC35 and chemiluminescent detection. For a gel shift assay, use Cat. No. OP09L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Application references	Original Clone Legros, Y., et al. 1994. Oncogene 9, 2071. Epitope Identification Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.

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