Millipore Sigma Vibrant Logo

OP43 Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
OP43
View Pricing & Availability

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Fe, HMMonoclonal Antibody

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
OP43-100UG
Retrieving availability...
—
Limited Availability Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 100 μg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      OP43-20UG
      Retrieving availability...
      —
      Limited Availability Limited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Plastic ampoule 20 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          Description
          OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
          Catalogue NumberOP43
          Brand Family Calbiochem®
          Application Data
          Detection of human p53 by immunoblotting. Sample: Whole cell lysates from A431 cells. Primary antibody: Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) (Cat. No. OP43) (1 μg/ml). Detection: chemiluminescence.
          References
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
          Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Negative controlSK-OV-3 cells or normal skin tissue
          Positive controlA431 cells or breast carcinoma tissue
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesImmunoprecipitation and Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Paraffin Sections
          Application NotesFrozen sections (1 µg/ml; see application references)
          Immunoblotting (0.1-1 µg/ml; see application references)
          Immunocytochemistry (1-2.5 µg/ml; see application references)
          Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references)
          Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
          Application CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenwild type, recombinant, human p53
          ImmunogenHuman
          EpitopeWithin amino acids 21-25 of human p53
          CloneDO-1
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Feline
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalog Number GTIN
          OP43-100UG 04055977227543
          OP43-20UG 04055977227123

          Documentation

          Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Certificates of Analysis

          TitleLot Number
          OP43

          References

          Reference overview
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
          Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

          Citations

          Title
        • Fang Zhang, et al. (2006) Ribosomal stress couples the unfolded protein response to p53-dependent cell cycle arrest. Journal of Biological Chemistry 281, 30036-30045.
        • Frank Staib, et al. (2005) The p53 Tumor Suppressor Network is a Key Responder to Microenvironmental Components of Chronic Inflammatory Stress. Cancer Research 65, 10255-10264.
        • Hazel E. Warburton, et al. (2005) p53 regulation and function in renal cell carcinoma. Cancer Research 65, 6498-6503.
        • Urs Hobom and Matthias Dobbelstein. (2004) E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection. Journal of Virology 78, 7685-7697.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-June-2008 JSW
          ApplicationFrozen sections (1 µg/ml; see application references)
          Immunoblotting (0.1-1 µg/ml; see application references)
          Immunocytochemistry (1-2.5 µg/ml; see application references)
          Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references)
          Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
          Application Data
          Detection of human p53 by immunoblotting. Sample: Whole cell lysates from A431 cells. Primary antibody: Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) (Cat. No. OP43) (1 μg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
          BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death.
          HostMouse
          Immunogen speciesHuman
          Immunogenwild type, recombinant, human p53
          EpitopeWithin amino acids 21-25 of human p53
          CloneDO-1
          IsotypeIgG2a
          Speciesfeline, human, not mouse, not rat
          Positive controlA431 cells or breast carcinoma tissue
          Negative controlSK-OV-3 cells or normal skin tissue
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
          Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
          Citation
        • Fang Zhang, et al. (2006) Ribosomal stress couples the unfolded protein response to p53-dependent cell cycle arrest. Journal of Biological Chemistry 281, 30036-30045.
        • Frank Staib, et al. (2005) The p53 Tumor Suppressor Network is a Key Responder to Microenvironmental Components of Chronic Inflammatory Stress. Cancer Research 65, 10255-10264.
        • Hazel E. Warburton, et al. (2005) p53 regulation and function in renal cell carcinoma. Cancer Research 65, 6498-6503.
        • Urs Hobom and Matthias Dobbelstein. (2004) E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection. Journal of Virology 78, 7685-7697.
          		•
          Application referencesImmunoprecipitation and Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.

          Related Products & Applications

          Related Products

          Catalog Number Description  
          OP43L Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Show Pricing & Availability

          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies