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FCMAB110P Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate detects level of phospho-ATM (Ser1981) & has been published & validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Apoptosis & Cancer Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: ATM, AT, TELO1, ATC, DKFZp781A0353, MGC74674, ATD, ATA, AT1, T-PLL, ATE, TEL1, ATDC, TPLL UniProt Number: Q13315
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FCMAB110P
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFCMPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberFCMAB110P
      DescriptionAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate
      OverviewN-terminal c-Myc, GST-tagged, recombinant human Cardiac Troponin I full length, expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
      Alternate Names
      • A-T, mutated
        AT mutated
        TEL1, telomere maintenance 1, homolog
        ataxia telangiectasia mutated
        ataxia telangiectasia mutated (includes complementation groups A, C and D)
        ataxia telangiectasia mutated protein
        human phosphatidylinositol 3-kinase homolog
        serine-protein kinase ATM
      Background InformationAtaxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
      References
      Product Information
      FormatPhycoerythrin
      HS Code3002 15 90
      Control
      • Irradiated HeLa cells
      PresentationPurified mouse monoclonal IgG1κ conjugated to phycoerythrin in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate detects level of phospho-ATM (Ser1981) & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to human ATM phosphorylated at Ser1981.
      EpitopePhosphorylated at and around Ser1981
      Clone10H11.E12
      HostMouse
      SpecificityAntibody recognizes ATM phosphorylated at Ser1981.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and mouse. Predicted to cross-react with rat based on sequence homology
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • ATM
      • AT
      • TELO1
      • ATC
      • DKFZp781A0353
      • MGC74674
      • ATD
      • ATA
      • AT1
      • T-PLL
      • ATE
      • TEL1
      • ATDC
      • TPLL
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13315 # Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function.
      SIZE: 3056 amino acids; 350644 Da
      SUBUNIT: Exists in monomeric and tetrameric state. Binds DNA ends, p53/TP53, ABL1, BRCA1, NBN/nibrin and TERF1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. Interacts with EEF1E1. This interaction, which takes place independently of TP53, is induced by DNA damage that may occur during genotoxic stress or cell growth. Interacts with DCLRE1C. Interacts with MYST1. Interacts with HTATIP.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasmic vesicle. Note=Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
      TISSUE SPECIFICITY: Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.DOMAIN:SwissProt: Q13315 The FATC domain is required for interaction with HTATIP.
      PTM: Phosphorylated by ARK5. Autophosphorylated on Ser-1981 upon DNA damage. & Acetylated by HTATIP upon DNA damage; which is required for autophosphorylation and subsequent activation.
      DISEASE: SwissProt: Q13315 # Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation. & Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients. & Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL). & Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
      SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 1 PI3K/PI4K domain.
      Molecular Weight~370 kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry with HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      FCMAB110P 04053252405143

      Documentation

      Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate Certificates of Analysis

      TitleLot Number
      Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE conjugate - NRG1677934 NRG1677934
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - 1990434 1990434
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - 2002202 2002202
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - 2029175 2029175
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - 2204976 2204976
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1741009 NG1741009
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1818624 NG1818624
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1871120 NG1871120
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1895075 NG1895075
      Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1947164 NG1947164
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      Alternative Format

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