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MABT1443-25UL Anti-phospho-Connexin 43 (Ser368) Antibody, clone 2C6

Anti-phospho-Connexin 43 (Ser368), clone 2C6, Cat. No. MABT1443, is a mouse monoclonal antibody that detects Connexin-43 phosphorylated on serine 368 and is tested for use in Western Blotting.

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MABT1443-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1443-25UL
      DescriptionAnti-phospho-Connexin 43 (Ser368) Antibody, clone 2C6
      Alternate Names
      • Gap junction alpha-1 protein
      • Connexin-43
      • Cx43
      • Gap junction 43 kDa heart protein
      Background InformationGap junction alpha-1 protein (UniProt: P17302; also known as Connexin-43, Cx43, Gap junction 43 kDa heart protein) is encoded by the GJA1 (also known as GJAL) gene (Gene ID: 2697) in human. Cx43 is a member of the gap junction family, which is composed of intercellular channels allowing direct cell-to-cell communication between neighboring cells via the exchange of small ions and metabolites. It is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is one of the most widely studied gap junction proteins that regulates N-cadherin expression during collective cell migration. It also acts as a negative regulator of bladder capacity by enhancing intercellular electrical and chemical transmission and thereby exsensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. It is also expressed in gap junctions of the cardiac ventricle and plays an essential role in gap junction communication in the ventricles. Cx43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Cx43 is phosphorylated at Ser-368 by PKCg (PRKCG) and this phosphorylation is reported to induce disassembly of gap junction plaques and inhibition of gap junction activity and triggers its internalization into small vesicles leading to proteasome-mediated degradation. (Ref.: Solan, JL., and Lampe, PD. (2020). Biomolecules. 10(12); 1596; Kotini, M., et al. (2018). Nature Commun. 9; article 3846).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho-Connexin 43 (Ser368), clone 2C6, Cat. No. MABT1443, is a mouse monoclonal antibody that detects Connexin-43 phosphorylated on serine 368 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis (WB): A representative lot of this antibody detected phospho-Connexin-43 (Ser368) in NRK cells. ( Solan, JL., and Lampe, PD. (2020). Biomolecules. 10(12); 1596).


      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 368 from the C-terminal, cytoplasmic domain of human Connexin 43.
      EpitopeC-terminal
      Clone2C6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2C6 is a mouse monoclonal antibody that detects Connexin 43 phosphorylated on serine 368.
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human, Bovine, Canine, Feline, Monkey, Porcine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GJA1
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~42 kDa observed; 43.01 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NRK cell lysate.

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Connexin 43 phosphorylated on serine 368 in lysates from untreated NRK cells and in NRK cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA; 25 nM; 40 min).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT1443-25UL 04065267060347