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MABN1813 Anti-sodium/hydrogen exchanger 3 Antibody, clone 3H3

Anti-NHE3, clone 3H3, Cat. No. MABN1813, is a highly specific mouse monoclonal antibody that targets Sodium/hydrogen exchanger 3 and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: SLC9A3, NHE3 UniProt Number: Q28362
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MABN1813
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, OpIHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1813
      DescriptionAnti-sodium/hydrogen exchanger 3 Antibody, clone 3H3
      Alternate Names
      • Sodium/hydrogen exchanger 3
      • Na(+)/H(+) exchanger 3
      • Solute carrier family 9 member 3
      Background InformationSodium/hydrogen exchanger 3 (UniProt: Q28362; also known as Na(+)/H(+) exchanger 3, NHE-3, Solute carrier family 9 member 3) is encoded by the SLC9A3 (also known as NHE3) gene in Didelphis virginiana (North American opossum). NHE-3 is a multi-pass membrane protein that is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. NHE-3 is predominantly expressed in kidney and gut, where it is found on the apical membranes of polarized epithelial cells and plays a central role in transepithelial reabsorption of sodium ions and secretion of hydrogen ions. Renal NHE-3 is essential in maintaining blood pressure and steady-state plasma sodium levels when dietary sodium chloride intake is modified. In the intestine epithelial cells, it localizes to the ileal brush border. Dysfunction of NHE-3 in intestine is associated with a variety of diarrheal diseases. HNE-3 can be phosphorylated by SGK1 at Ser669 that increases its abundance at the cell membrane. (Ref.: D Souza, S et al. (1998). J. Biol. Chem. 273(4); 2035 2043; Fenton, RA et al (2017). Kidney Int. doi: 10.1016/j.kint.2017.02.001. [Epub ahead of print]).
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NHE3, clone 3H3, Cat. No. MABN1813, is a highly specific mouse monoclonal antibody that targets Sodium/hydrogen exchanger 3 and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NHE3 in human kidney and human stomach tissue sections.

      Western Blotting Analysis: A representative lot detected NHE3 in Western Blotting applications (Wang, D., et. al. (2006). Am J Physiol Renal Physiol. 290(2):F428-37; Queiroz-Leite, G.D., et. al. (2011). Biochem Biophys Res Commun. 409(3):470-6; Goyal, S., et. al. (2005). Am J Physiol Renal Physiol. 288(3):F530-8; Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58; Di Sole, F., et. al. (2008). J Cell Physiol. 216(1):221-33; Baum, M., et. al. (2012). Am J Physiol Renal Physiol. 303(11):F1495-502; Lessa, L.M., et. al. (2012). Am J Physiol Renal Physiol. 303(10):F1399-408).

      Immunohistochemistry Analysis: A representative lot detected NHE3 in Immunohistochemistry applications (Pao, A.C., et. al. (2010). Am J Physiol Renal Physiol. 299(6):F1496-506).

      Immunoprecipitation Analysis: A representative lot detected NHE3 in Immunoprecipitation applications (Girardi, A.C., et. al. (2004). Am J Physiol Renal Physiol. 287(5):C1238-45).
      Biological Information
      ImmunogenMBP-conjugated recombinant fragment corresponding to 356 amino acids from the C-terminal half of Opossum sodium/hydrogen exchanger 3.
      Clone3H3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone CH3 detects Sodium/hydrogen exchanger 3 in human, opossum, and rat kidney cells. It targets an epitope within 356 amino acids from the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Opossum
      Species Reactivity NoteHuman, Mouse, Opossum, Rat. Predicted to react with Porcine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • SLC9A3
      • NHE3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~75 kDa observed; 94.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in COS-7 cells transfected with wild type NHE3 plasmid DNA.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected NHE3 in lysates from COS-7 cells transfected with wild type NHE3 plasmid DNA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN1813 04054839396731

      Documentation

      Anti-sodium/hydrogen exchanger 3 Antibody, clone 3H3 Certificates of Analysis

      TitleLot Number
      Anti-sodium/hydrogen exchanger 3, clone 3H3 - 3174769 3174769
      Anti-sodium/hydrogen exchanger 3, clone 3H3 - 3683144 3683144
      Anti-sodium/hydrogen exchanger 3, clone 3H3 - 3762445 3762445
      Anti-sodium/hydrogen exchanger 3, clone 3H3 - 3899604 3899604
      Anti-sodium/hydrogen exchanger 3, clone 3H3 - 4010930 4010930
      Anti-sodium/hydrogen exchanger 3, clone 3H3 - 4042771 4042771
      Anti-sodium/hydrogen exchanger 3, clone 3H3 - 4123925 4123925
      Anti-sodium/hydrogen exchanger 3, clone 3H3 Monoclonal Antibody Q2959745
      Anti-sodium/hydrogen exchanger 3, clone 3H3 Monoclonal Antibody 3128827
      Anti-sodium/hydrogen exchanger 3, clone 3H3 Monoclonal Antibody 3062993