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382061 Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem

Membrane-permeable, adenine-thymine-specific fluorescent stain.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258

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382061
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
23491-45-4C₂₅H₂₄N₆O · 3HCl

Pricing & Availability

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382061-100MG
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      382061-1GM
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          382061-250MG
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              Description
              OverviewMembrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.
              Catalogue Number382061
              Brand Family Calbiochem®
              Synonyms2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
              References
              ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
              Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
              Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
              Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
              Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
              Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
              Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
              Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
              Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
              Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.
              Product Information
              CAS number23491-45-4
              ATP CompetitiveN
              FormYellow-green solid
              Hill FormulaC₂₅H₂₄N₆O · 3HCl
              Chemical formulaC₂₅H₂₄N₆O · 3HCl
              ReversibleN
              Structure formula ImageStructure formula Image
              Quality LevelMQ100
              Applications
              Biological Information
              Primary TargetAdenine-thymine-specific fluorescent stain
              Purity≥98% by HPLC
              Physicochemical Information
              Cell permeableY
              Emission max.
              Excitation max.
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSSM1140500
              Safety Information
              R PhraseR: 36/37/38-40

              Irritating to eyes, respiratory system and skin.
              Limited evidence of a carcinogenic effect.
              S PhraseS: 22-26-36

              Do not breathe dust.
              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Wear suitable protective clothing.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Carcinogenic / Teratogenic
              Storage +2°C to +8°C
              Protect from Light Protect from light
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Catalog Number GTIN
              382061-100MG 04055977213065
              382061-1GM 04055977213058
              382061-250MG 04055977213072

              Documentation

              Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem SDS

              Title

              Safety Data Sheet (SDS) 

              Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem Certificates of Analysis

              TitleLot Number
              382061

              References

              Reference overview
              Werstuck, G., and Green, M.R. 1998. Science 282, 296.
              Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
              Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
              Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
              Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
              Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
              Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
              Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
              Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
              Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision07-June-2015 JSW
              Synonyms2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
              DescriptionMembrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5' untranslated region (UTR) to control expression of transfected gene constructs.
              FormYellow-green solid
              CAS number23491-45-4
              RTECSSM1140500
              Chemical formulaC₂₅H₂₄N₆O · 3HCl
              Structure formulaStructure formula
              Purity≥98% by HPLC
              SolubilityH₂O (20 mg/ml). Precipitates in phosphate buffered solutions
              Storage Protect from light
              +2°C to +8°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.
              Toxicity Carcinogenic / Teratogenic
              ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
              Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
              Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
              Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
              Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
              Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
              Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
              Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
              Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
              Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.

              Related Products & Applications

              Alternative Products

              Catalog Number Description  
              382065 Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem Show Pricing & Availability

              Categories

              Life Science Research > Inhibitors and Biochemicals > Biochemicals > Dyes, Stains and Probes > DNA-Binding Dyes