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371207 Dkk-1, Mouse, Recombinant

Dkk-1, Mouse, Recombinant , consists of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

Dkk-1, Mouse, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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371207
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Overview

Replacement Information

Key Specifications Table

Purity
≥95% by SDS-PAGE

Pricing & Availability

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371207-10UG
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      Description
      OverviewRecombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND50 = 0.02-0.1 µg/ml.
      Catalogue Number371207
      Brand Family Calbiochem®
      References
      ReferencesPinzone, J.J., et al. 2009. Blood 113, 517.
      Li, J., et al. 2006. Bone 36, 754.
      Morvan, F., et al. 2006. J. Bone Miner. Res. 21, 934.
      Niehrs, C., 2006. Oncogene 25, 7469.
      Verani, R., et al. 2006. J. Neurochem. 101, 242.
      Cappuccio, I., et al. 2005. J. Neurosci. 25, 2647.
      Kemp, C., et al. 2005. Dev. Dyn. 233, 1064.
      Adamska, M., et al. 2004. Dev. Biol. 272, 134.
      Bullock, C.M., et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B., et al. 2002. Nature 417, 664.
      Mao, B., et al. 2001. Nature 411, 321.
      Glinka, A., et al. 1998. Nature 391, 357.
      Product Information
      FormLiquid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      371207-10UG 04055977191790

      Documentation

      Dkk-1, Mouse, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      Dkk-1, Mouse, Recombinant Certificates of Analysis

      TitleLot Number
      371207

      References

      Reference overview
      Pinzone, J.J., et al. 2009. Blood 113, 517.
      Li, J., et al. 2006. Bone 36, 754.
      Morvan, F., et al. 2006. J. Bone Miner. Res. 21, 934.
      Niehrs, C., 2006. Oncogene 25, 7469.
      Verani, R., et al. 2006. J. Neurochem. 101, 242.
      Cappuccio, I., et al. 2005. J. Neurosci. 25, 2647.
      Kemp, C., et al. 2005. Dev. Dyn. 233, 1064.
      Adamska, M., et al. 2004. Dev. Biol. 272, 134.
      Bullock, C.M., et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B., et al. 2002. Nature 417, 664.
      Mao, B., et al. 2001. Nature 411, 321.
      Glinka, A., et al. 1998. Nature 391, 357.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-Sepmtember-2011 RFH
      DescriptionRecombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND50 = 0.02-0.1 µg/ml.
      FormLiquid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Biological activityND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.
      SolubilityReconstitute to 100 µg/ml in PBS containing at least 0.1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesPinzone, J.J., et al. 2009. Blood 113, 517.
      Li, J., et al. 2006. Bone 36, 754.
      Morvan, F., et al. 2006. J. Bone Miner. Res. 21, 934.
      Niehrs, C., 2006. Oncogene 25, 7469.
      Verani, R., et al. 2006. J. Neurochem. 101, 242.
      Cappuccio, I., et al. 2005. J. Neurosci. 25, 2647.
      Kemp, C., et al. 2005. Dev. Dyn. 233, 1064.
      Adamska, M., et al. 2004. Dev. Biol. 272, 134.
      Bullock, C.M., et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B., et al. 2002. Nature 417, 664.
      Mao, B., et al. 2001. Nature 411, 321.
      Glinka, A., et al. 1998. Nature 391, 357.