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PMEF-NL-P1 EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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PMEF-NL-P1
1 vial  1x106 cells
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      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberPMEF-NL-P1
      Trade Name
      • Embryomax
      • Specialty Media
      DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
      OverviewPMEF-NL-P1 are low passage neomycin resistant Primary Mouse Embryo Fibroblasts (PMEFs) isolated from FVB mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.
      Alternate Names
      • EmbryoMax® Primary Mouse Embryonic Fibroblasts
      Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
      References
      Product Information
      PresentationProvided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.
      Quality LevelMQ100
      Applications
      ApplicationPMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • PMEFs
      • Induced Pluripotent Stem Cells
      Cell Line Type
      • Primary Mouse Embryo Fibroblasts
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAfter receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
      Packaging Information
      Material Size1 vial
      Material Package1x106 cells
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PMEF-NL-P1 04053252851810

      Related Products & Applications

      Alternative Format

      Catalog Number Description  
      PMEF-NL EmbryoMax® Primary Mouse Embryonic Fibroblasts Show Pricing & Availability

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      EmbryoMax®

      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Primary Mouse Embryo Fibroblast (PMEF) Feeder Cells