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324716 Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli

Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli, CAS 59793-96-3, catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to Ser or Thr.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: O-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosaminohydrolase, O-Glycosidase

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324716
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      Description
      OverviewRecombinant, Streptococcus pneumoniae Endo-α-N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides. For carbohydrates containing sialic acid or fucose, pretreatment with neuraminidase or fucosidase is required.
      Note: 1 mU = 1 milliunit.
      Catalogue Number324716
      Brand Family Calbiochem®
      SynonymsO-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosaminohydrolase, O-Glycosidase
      References
      ReferencesWang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
      Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
      Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
      Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
      Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.
      Product Information
      CAS number59793-96-3
      Activity≥1 unit/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside per min at 37°C, pH 5.0.
      EC number3.2.1.97
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥10 units/mg protein
      Physicochemical Information
      ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidase, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      324716-50MIU 04055977215977

      Documentation

      Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      324716

      References

      Reference overview
      Wang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
      Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
      Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
      Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
      Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      SynonymsO-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosaminohydrolase, O-Glycosidase
      DescriptionRecombinant, Streptococcus pneumoniae Endo-α-N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides.
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, pH 7.5.
      Recommended reaction conditions
      Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli Note: This protocol is provided only as a general guidline. Researchers should standardize this assay for their own specific needs and should consult published literature. Endo-α-N-acetylgalactosaminidase cleaves only unsubstituted Galβ1,3GalNAc disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides. Substitutions such as sialic acid, galactose, fucose, or N-acetylglucosamine must first be removed with the appropriate exoglycosidase prior to treatment with Endo-α-N-acetylgalactosaminidase. At a minimum, a neuraminidase (e.g. α2-3,6,8-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, Cat. No. 480716) is almost always required to remove sialic acid. Required Reagents • 250 µM substrate (p-nitrophenol-2-acetamido-2-deoxy-3-O-(β-Dgalactopyranosyl)-α-D-galactopyranoside (Cat. No. 575303; also called T antigen) in 5 mM Sodium phosphate, pH 5.0 • 1 M Na2CO3 • Spectrophotometer Protocol 1. Adjust the spectrophotometer to read 400 nm. 2. Add 0.4 ml of substrate solution to two tubes and warm to 37°C. 3. Add 5 µl of enzyme to one tube and mix. Continue incubating at 37°C. 4. Following a 5 minute incubation, add 0.6 ml of 1 M Na2CO3 to both tubes. 5. Blank the spectrophotometer using the control tube without enzyme. 6. Read the absorbance of the sample at 400 nm. 7. Calculate the units: Units/ml = A/18.3 TV A = absorbance of the sample T = time in min V = volume of enzyme in ml 18.3 = millimolar extinction coefficient of pNP at pH >10 Note: Endo-α-N-acetylgalactosaminidase cleaves methylumbelliferyl-α-D-Nacetylgalactosamide (but not pNP-α-D-N-acetylgalactosamide) at 0.1% the rate of p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside. Assay for Deglycosylation Required Reagents • Endo-α-N-acetylgalactosaminidase • 5X assay buffer: 250 mM sodium phosphate, pH 5.0 • sterile dH2O • α2-3,6,8-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, (Cat. No. 480716) • glycoprotein sample • SDS-PAGE system Protocol 1. Add up to 100 µg of glycoprotein to a tube. 2. Add water to 13 µl and then add 4 µl of 5X assay buffer. 3. Add 1 µl of α2-3,6,8-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli. 4. Add 2 µl of Endo-α-N-acetylgalactosaminidase 5. Incubate at 37°C for 1 h. Cleavage may be monitored by SDS-PAGE if the size differential between the native and the de-O-glycosylated protein is sufficient for detection.
      CAS number59793-96-3
      EC number3.2.1.97
      ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidase, proteases: none detected
      Specific activity≥10 units/mg protein
      Activity≥1 unit/ml
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside per min at 37°C, pH 5.0.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesWang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
      Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
      Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
      Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
      Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.