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14-743 GCK Protein, active, 10 µg

Active, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

GCK Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-743
10 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-743
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionGCK Protein, active, 10 µg
      OverviewN-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells.
      Alternate Names
      • germinal center kinase
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceRecombinant protein expressed in Sf21 cells
      Specific Activity2337 U/mg, where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.
      Gene Symbol
      • MAP4K2
      • GCK
      • RAB8IP
      • BL44
      Protein TargetGCK
      Purification MethodPurified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining.
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q12851 # Enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.
      COFACTOR: Magnesium.
      SIZE: 819 amino acids; 91586 Da
      SUBUNIT: Interacts with TRAF2, MAP3K1 and RAB8A.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Basolateral cell membrane; Peripheral membrane protein (By similarity). Golgi apparatus membrane; Peripheral membrane protein (By similarity).
      TISSUE SPECIFICITY: Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
      SIMILARITY: SwissProt: Q12851 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CNH domain. & Contains 1 protein kinase domain.
      Molecular Weight80.6 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
      Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-743 04053252277603

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      Alternative Packsize

      Catalog Number Description  
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      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases