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SCC188 GN11 Mouse GnRH Neuronal Cell Line

The GN11 mouse neuronal cell line is an immortalized cell line established as a model of GnRH-secreting neurons.

SCC188
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC188
      DescriptionGN11 Mouse GnRH Neuronal Cell Line
      OverviewSource:
      The GN11 cell line was derived from a transgenic mouse expressing Simian virus-40 (SV40) large T antigen driven by the human GnRH promoter (3). An olfactory bulb tumor from one male GnRH promoter-SV40T fusion-expressing transgenic mouse were dispersed and the GN11 cell line was established from serial dilution and cloning from the primary cells.
      Background InformationGonadotropin-releasing hormone (GnRH) is important for control of reproduction in all mammals, acting to stimulate the synthesis and secretion of follicle-stimulating hormone and luteinizing hormone from the pituitary gland (1).GnRH is secreted by specialized neurons that migrate from the olfactory bulb to the brain during embryonic development. GnRH neurons are highly sensitive to glucose and insulin, suggestive of their physiological role in sensing the metabolic status in preparation for reproduction (2). Defects in GnRH neuronal function result in subfertility and developmental abnormalities. The scattered distribution and scarcity of GnRH neurons in the brain complicate the in vivo study of these cells and necessitate the utilization of robust in vitro cell models.

      The GN11 mouse neuronal cell line is an immortalized cell line established as a model of GnRH-secreting neurons (3). In addition to GnRH expression, GN11 cells display neuron-specific markers including microtubule-associated protein 2 (MAP2) and Tau (4). GN11 cells express insulin receptor (5) and are responsive to steroid hormones (6). The GN11 GnRH-secreting neuronal cell line has proven a valuable system for exploring the cellular mechanisms of neuroendocrine reproductive physiology.

      References:
      1. Forni PE, Wray S (2012) Mol Neurobiol 46(2): 349-360.
      2. Roland AV, Moenter SM (2011) Trends Endocrinol Metab 22(11): 443-449.
      3. Radovick S et al. (1991) Proc Natl Acad Sci USA 88(8): 3402-3406.
      4. Zhen S et al. (1997) J Biol Chem 272 (19): 12620-12625.
      5. DiVall SA, et al. (2007) Mol Cell Endocrinol 270(1–2):64–72.
      6. Novaira HJ, et al. (1994) Horm Behav 28(4):520-529.
      References
      Product Information
      Applications
      ApplicationThe GN11 mouse neuronal cell line is an immortalized cell line established as a model of GnRH-secreting neurons.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Neural Lineage Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      SCC188 04061841031697