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361526 GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli

GSK-3β, Rabbit Skeletal MuscleHis-Tag®, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: GSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli

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361526
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361526-5KU
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      Description
      OverviewRecombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Dual specificity kinase. One of several protein kinases that phosphorylates glycogen synthase. Other substrates include p90rsk, Tau, c-Jun, and CREB. Plays a key role in Wnt and insulin signaling.
      Note: 1 KU = 1000 units.
      Catalogue Number361526
      Brand Family Calbiochem®
      SynonymsGSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
      References
      ReferencesDajani, R., et al. 2001. Cell 105, 721.
      Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
      Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
      Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
      Wang, Q.M., et al. 1994. Biochemistry 33, 143.
      deGroot, R.P., et al. 1993. Oncogene 8, 841.
      Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.
      Product Information
      Activity≥250,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.
      FormLiquid
      FormulationIn 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5
      Quality LevelMQ100
      Applications
      ApplicationGSK-3β, Rabbit Skeletal MuscleHis-Tag®, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.
      Biological Information
      Physicochemical Information
      ContaminantsDNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      361526-5KU 04055977192933

      Documentation

      GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      361526

      References

      Reference overview
      Dajani, R., et al. 2001. Cell 105, 721.
      Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
      Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
      Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
      Wang, Q.M., et al. 1994. Biochemistry 33, 143.
      deGroot, R.P., et al. 1993. Oncogene 8, 841.
      Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-September-2007 RFH
      SynonymsGSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
      DescriptionRecombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Glycogen synthase kinase 3β is a monomeric protein whose activity is independent of second messengers such as cAMP or IP3. Recent studies demonstrate that, like the dual specificity MAP kinase, it autophosphorylates on Ser, Thr, and Tyr. It phosphorylates several exogenous substrates, including protein phosphatase 1, the regulatory domain of protein kinase A, myelin basic protein, protein phosphatase inhibitor 2, c-jun, c-myb, c-myc, CREB, CREM, p90rsk, and microtubule-associated tau protein only on Ser and/or Thr, but not on Tyr residues. Plays a key role in Wnt and insulin signaling.
      FormLiquid
      FormulationIn 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5
      Recommended reaction conditions20 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 5 mM DTT, 200 µM ATP, 30°C. Optimal incubation times and enzyme concentrations must be determined empirically for each substrate.
      ContaminantsDNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation.
      Activity≥250,000 units/ml
      Unit definitionOne unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDajani, R., et al. 2001. Cell 105, 721.
      Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
      Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
      Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
      Wang, Q.M., et al. 1994. Biochemistry 33, 143.
      deGroot, R.P., et al. 1993. Oncogene 8, 841.
      Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.