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14-306 GSK3β Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.

GSK3β Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-306
10 µg  Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-306
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionGSK3β Protein, active, 10 µg
      OverviewN-terminal His6-tagged recombinant human GSK3β with an H350L mutation
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryGlycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]
      Gene Symbol
      • GSK3B
      Protein TargetGSK3β
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P49841 # Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
      SIZE: 420 amino acids; 46744 Da
      SUBUNIT: Monomer (By similarity). Interacts with NIN and CABYR.
      TISSUE SPECIFICITY: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
      PTM: Phosphorylated by AKT1 and ILK1.
      SIMILARITY: SwissProt: P49841 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain.
      Molecular Weight51kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of Phospho-Glycogen Synthase Peptide-2
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-306 04053252409486

      Documentation

      GSK3β Protein, active, 10 µg SDS

      Title

      Safety Data Sheet (SDS) 

      GSK3β Protein, active, 10 µg Certificates of Analysis

      TitleLot Number
      GSK-3#946;, active - D8JN013U-T D8JN013U-T
      GSK-3#946;, active - D8JN013U-V D8JN013U-V
      GSK-3#946;, active - D8JN013U-W D8JN013U-W
      GSK-3#946;, active - D8JN013U-X D8JN013U-X
      GSK-3#946;, active - D8JN013U-Y D8JN013U-Y
      GSK-3#946;, active - D8JN013U-Z D8JN013U-Z
      GSK-3ß, active - D8JN013UAA D8JN013UAA
      GSK-3β, active - D8JN013FU D8JN013FU
      GSK-3β, active - WAA0024-I WAA0024-I
      GSK‐3β ,active - D8JN013UA-A D8JN013UA-A

      References

      Reference overviewApplicationPub Med ID
      Ethanol impairs insulin-stimulated neuronal survival in the developing brain: role of PTEN phosphatase
      Xu, J., et al
      J Biol Chem, 278:26929-37 (2003)  2003

      		Kinase Assay12700235 12700235
      Protein kinase involved in lung injury susceptibility: Evidence from enzyme isoform genetic knockout and in vivo inhibitor treatment
      Wainwright, M. S., et al
      Proc Natl Acad Sci U S A, 100:6233-8 (2003)  2003

      		Activation Assay12730364 12730364
      The alpha-isoform of glycogen synthase kinase-3 from rabbit skeletal muscle is inactivated by p70 S6 kinase or MAP kinase-activated protein kinase-1 in vitro.
      Sutherland, C and Cohen, P
      FEBS Lett., 338: 37-42 (1994)  1994

      Show Abstract       		8307153 8307153
      Regulation and functions of the glycogen synthase kinase-3 subfamily.
      Woodgett, J R
      Semin. Cancer Biol., 5: 269-75 (1994)  1994

      Show Abstract       		7803763 7803763

      FAQ

      QuestionAnswer
      Why is it important to shake the reaction mixture during the incubations?It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.

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      Alternative Packsize

      Catalog Number Description  
      14-306M GSK3β Protein, active, 250 µg Show Pricing & Availability

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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases