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AP112B Goat Anti-Human IgG Antibody, biotin conjugate

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AP112B
2 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP112B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Human IgG Antibody, biotin conjugate
      Background InformationIgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
      References
      Product Information
      FormatBiotin
      PresentationPurified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesEIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
      Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
      Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
      Flow cytometry: 1:200-1:1,000.
      Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPrepared from purified human IgG.
      HostGoat
      SpecificityThe antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
      Packaging Information
      Material Size2 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AP112B 04053252478307

      Documentation

      Goat Anti-Human IgG Antibody, biotin conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Goat Anti-Human IgG Antibody, biotin conjugate Certificates of Analysis

      TitleLot Number
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED 2475585
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED SECONDARY ANTIBODY - 2117136 2117136
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 2492061 2492061
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3174897 3174897
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3212810 3212810
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3385800 3385800
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3427264 3427264
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3464588 3464588
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3488179 3488179
      GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3574525 3574525

      References

      Reference overviewPub Med ID
      Herpes simplex virus type-2 specific glycoprotein G-2 immunomagnetically captured from HEp-2 infected tissue culture extracts.
      Charles R Clavet, Aaron B Margolin, Patrick M Regan
      Journal of virological methods  119  121-8  2004

      Show Abstract
      15158593 15158593

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies