Millipore Sigma Vibrant Logo

100327 Hydrochloric acid in ethanol

for microscopy

Hydrochloric acid in ethanol MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Hydrogen chloride solution

View Products on Sigmaaldrich.com
100327
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
1003271000
Retrieving availability...
—
Limited Availability Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic bottle 1 l
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      1003275000
      Retrieving availability...
      —
      Limited Availability Limited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Plastic container 5 l
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          Description
          Catalogue Number100327
          SynonymsHydrogen chloride solution
          DescriptionHydrochloric acid in ethanol
          OverviewHydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Cat.no.100327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
          Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3824 99 92
          Quality LevelMQ400
          Applications
          Applicationfor microscopy
          Biological Information
          Physicochemical Information
          Density0.90 g/cm3 (20 °C)
          Flash point21 °C
          pH value1.0 (H₂O, 20 °C)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Pictogram(s)
          Hazard Statement(s)H225: Highly flammable liquid and vapour.
          H290: May be corrosive to metals.
          H319: Causes serious eye irritation.
          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
          P233: Keep container tightly closed.
          P234: Keep only in original packaging.
          P240: Ground and bond container and receiving equipment.
          P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
          P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
          P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
          Signal WordDanger
          Storage class3 Flammable liquids
          WGKWGK 1 slightly hazardous to water
          Disposal3
          Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
          Safety Information
          Categories of dangerflammable
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +2°C to +30°C.
          Packaging Information
          Transport Information
          Declaration (railroad and road) ADR, RIDUN 2924 , 3 (8), II
          Declaration (transport by air) IATA-DGRUN 2924 , 3 (8), II
          Declaration (transport by sea) IMDG-CodeUN 2924 , 3 (8), II, Segregation Group: 1 (Acids)
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalog Number GTIN
          1003271000 04022536004907
          1003275000 04022536004921

          Related Products & Applications

          Accessories (Secondary)

          Catalog Number Description  
          109215 Ziehl-Neelsen carbol-fuchsin solution Show Pricing & Availability

          Related Products By: Application Facete

          Counter Staining
          Bacteria Staining
          Acid-Fast Bacteria Staining

          Categories

          IVD/OEM Materials & Reagents > Microscopy > Universal Reagents > Auxiliaries for Microscopy
          IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining