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400051 Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem

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MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
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400051
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
31282-04-9C₂₀H₃₇N₃O₁₃

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      400051-5MU
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          Description
          OverviewUnique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent.
          Bioassay: 350-450 units/mg Hygromycin B. 106 units is equivalent to ~900 mg.
          Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units.
          Catalogue Number400051
          Brand Family Calbiochem®
          References
          ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
          Hamada, W., et al. 1994. Curr. Genetics 26, 251.
          Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
          Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
          Sugimoto, K., et al. 1994. Plant J. 5, 863.
          Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
          Gaken, J., et al. 1992. Biotechniques 13, 32.
          Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
          Ma, H., et al. 1992. Gene 117, 161.
          MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
          Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
          Bilang, R., et al. 1991. Gene 100, 247.
          Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
          Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
          Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
          MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
          Zhou, J., et al. 1991. Gene 107, 307.
          Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
          Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
          Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
          Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
          Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
          Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
          Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
          Kronstad, J.W., et al. 1989. Gene 79, 97.
          Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
          Cullen, D., et al. 1987. Gene 57, 21.
          Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
          Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
          Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
          Product Information
          CAS number31282-04-9
          FormAmber to brown liquid
          Hill FormulaC₂₀H₃₇N₃O₁₃
          Chemical formulaC₂₀H₃₇N₃O₁₃
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Biological Information
          Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
          Primary Targetgrowth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
          Secondary targetprotein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA
          Purity>85% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSWK2130000
          Safety Information
          R PhraseR: 26/27/28-37/38-41

          Very toxic by inhalation, in contact with skin and if swallowed.
          Irritating to respiratory system and skin.
          Risk of serious damage to eyes.
          S PhraseS: 23-26-36/37/39-45

          Do not breathe fumes.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic & Carcinogenic / Teratogenic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

          Note: This product is highly toxic. Read enclosed MSDS before handling.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalog Number GTIN
          400051-1MU 04055977212174
          400051-5MU 07790788049966

          Documentation

          Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem Certificates of Analysis

          TitleLot Number
          400051

          References

          Reference overview
          Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
          Hamada, W., et al. 1994. Curr. Genetics 26, 251.
          Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
          Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
          Sugimoto, K., et al. 1994. Plant J. 5, 863.
          Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
          Gaken, J., et al. 1992. Biotechniques 13, 32.
          Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
          Ma, H., et al. 1992. Gene 117, 161.
          MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
          Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
          Bilang, R., et al. 1991. Gene 100, 247.
          Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
          Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
          Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
          MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
          Zhou, J., et al. 1991. Gene 107, 307.
          Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
          Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
          Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
          Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
          Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
          Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
          Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
          Kronstad, J.W., et al. 1989. Gene 79, 97.
          Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
          Cullen, D., et al. 1987. Gene 57, 21.
          Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
          Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
          Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.

          Brochure

          Title
          Antibiotics Profiler
          G418/Hygromycin B Flyer
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-January-2018 JSW
          DescriptionHygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).

          Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).

          The analytical data listed below will vary from lot to lot.

          Bioassay: 350-450 U/mg
          Concentration: 444,600 U/ml; 394 mg Hygromycin B/ml
          Density: 1140-1155 mg/ml
          % solids: 34.6%
          Fill volumes:
          100,000 U = 225 µl
          1,000,000 U = 2.25 ml
          10,000,000 U = 22.5 ml
          250,000 U = 562 µl
          5,000,000 U = 11.25 ml
          FormAmber to brown liquid
          Recommended reaction conditions
          Hygromycin B is not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 µm filter before use. When using this product for mammalian cell selection the working concentration is generally between 50 µg/ml and 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type. For researchers interested in understanding how the concentration in mg/ml can be calculated from the activity in U/ml we provide the following example as a guideline. For the 1,000,000 Unit size: 1,000,000 Units at 438,440 U/ml

          Figure 1: Calculations
          CAS number31282-04-9
          RTECSWK2130000
          Chemical formulaC₂₀H₃₇N₃O₁₃
          Structure formulaStructure formula
          Purity>85% by HPLC
          Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

          Note: This product is highly toxic. Read enclosed MSDS before handling.
          Toxicity Highly Toxic & Carcinogenic / Teratogenic
          Merck USA index14, 4852
          ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
          Hamada, W., et al. 1994. Curr. Genetics 26, 251.
          Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
          Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
          Sugimoto, K., et al. 1994. Plant J. 5, 863.
          Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
          Gaken, J., et al. 1992. Biotechniques 13, 32.
          Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
          Ma, H., et al. 1992. Gene 117, 161.
          MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
          Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
          Bilang, R., et al. 1991. Gene 100, 247.
          Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
          Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
          Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
          MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
          Zhou, J., et al. 1991. Gene 107, 307.
          Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
          Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
          Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
          Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
          Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
          Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
          Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
          Kronstad, J.W., et al. 1989. Gene 79, 97.
          Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
          Cullen, D., et al. 1987. Gene 57, 21.
          Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
          Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
          Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.