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14-630 KDR Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant, human KDR amino acids 790-end,
for use in Kinase Assays.

KDR Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-630
10 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-630
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionKDR Protein, active, 10 µg
      Overviewhuman KDR expressed by baculovirus in Sf21 insect cells
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant, human KDR amino acids 790-end,
      for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceN-terminal His6-tagged, recombinant, human KDR amino acids 790-end
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • KDR
      • VEGFR-2
      • VEGFR2
      • CD309
      • FLK1
      • VEGFR
      Protein TargetKDR
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1356 amino acids; 151527 Da
      SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight67.9kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of myelin basic protein (MBP)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-630 04053252277849

      Documentation

      KDR Protein, active, 10 µg SDS

      Title

      Safety Data Sheet (SDS) 

      KDR Protein, active, 10 µg Certificates of Analysis

      TitleLot Number
      KDR, active - D7SN051U-O D7SN051U-O
      KDR, active - D7SN051U-P D7SN051U-P

      FAQ

      QuestionAnswer
      Why is it important to shake the reaction mixture during the incubations?It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.
      What type of buffer is best for lysis when attempting to preserve a protein?The best buffer is Buffer A. It is composed of 50mM Tris/HCl, pH 7.5, 1mM EGTA, 1mM EDTA, 1mM Sodium Orthovanadate (activated), 10mM Na B-glycerolphosphate, 50mM NaF, 5mM Na pyrophosphate, 1% w/v Triton X-100, 0.1% w/v 2-mercaptoethanol, 1ug/ml each of aprotinin, leupeptin and pepstatin, 1mM PMSF and 1mM microcystin (this is optional). QS with water. You may store Buffer A in aliquots at -20°C for 6 months.

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      Alternative Packsize

      Catalog Number Description  
      14-630M KDR Protein, active, 250 µg Show Pricing & Availability

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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases