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509306 Late Endosome Trafficking Inhibitor, EGA - CAS 415687-81-9 - Calbiochem

A cell-permeable compound that prevents various bacterial toxins from exerting their cytotoxic activity by blocking endosomal trafficking/maturation into acidified late endosomes.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 4-Bromobenzaldehyde-N-(2,6-dimethylphenyl)semicarbazone, (E)-2-(4-Bromobenzylidene)-N-(2,6-dimethylphenyl)hydrazinecarboxamide

Primary Target: host-membrane trafficking
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509306
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
415687-81-9C₁₆H₁₆BrN₃O

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      		 Glass bottle 25 mg
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      Description
      OverviewA cell-permeable semicabazone compound that prevents B. anthrax Lethal toxin/LT (EC50 = 1.7 µM in RAW264.7 cultures; ECmax = 6 µM in murine BMDM cultures), diphtheria toxin/DT, P. aeruginosa ExoA, and H. ducreyi cytolethal distending toxin (Hd-CDT) from exerting their cytotoxic activity, but not endocytic uptake, by blocking endosomal trafficking/maturation into acidified late endosomes, while exhibiting no cytoprotective efficacy against ricin & E. coli Ec-CDT that do not require acidified endosomes for intoxication. Likewise, EGA is also shown to inhibit low-pH-dependent viral infectivity by Armstrong strain of LCMV, A/WSN/33 strain of influenza virus, but not low-pH-independent infection of Nipah virus NiV or MLV. Although the exact molecular target remains unknown, EGA does not act as a pH neutralizing agent, nor does it inhibit endosomal recycling or trafficking to phagocytic compartments.
      Catalogue Number509306
      Brand Family Calbiochem®
      Synonyms4-Bromobenzaldehyde-N-(2,6-dimethylphenyl)semicarbazone, (E)-2-(4-Bromobenzylidene)-N-(2,6-dimethylphenyl)hydrazinecarboxamide
      References
      ReferencesGillespie, E.J., et al. 2013. Proc. Natl. Acad. Sci. USA 110, E4904.
      Product Information
      CAS number415687-81-9
      FormOff-white powder
      Hill FormulaC₁₆H₁₆BrN₃O
      Chemical formulaC₁₆H₁₆BrN₃O
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targethost-membrane trafficking
      Purity≥93% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      5.09306.0001 04055977241822

      Documentation

      Late Endosome Trafficking Inhibitor, EGA - CAS 415687-81-9 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Late Endosome Trafficking Inhibitor, EGA - CAS 415687-81-9 - Calbiochem Certificates of Analysis

      TitleLot Number
      509306

      References

      Reference overview
      Gillespie, E.J., et al. 2013. Proc. Natl. Acad. Sci. USA 110, E4904.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-March-2014 JSW
      Synonyms4-Bromobenzaldehyde-N-(2,6-dimethylphenyl)semicarbazone, (E)-2-(4-Bromobenzylidene)-N-(2,6-dimethylphenyl)hydrazinecarboxamide
      DescriptionA cell-permeable semicarbazone compound that prevents B. anthrax Lethal toxin/LT (Protective Antigen/PA & LF/Lethal Factor complex; EC50 = 1.7 µM in RAW264.7 cultures; ECmax = 6 µM in murine BMDM cultures), diphtheria toxin (DT), LF-DT A chain fusion LFnDTA/PA complex, P. aeruginosa ExoA, and H. ducreyi cytolethal distending toxin (Hd-CDT) from exerting their cytotoxic activity, but not endocytic uptake, by blocking endosomal trafficking/maturation into acidified late endosomes, while exhibiting no cytoprotective efficacy against toxins that do not require acidified endosomes for intoxication, such as ricin & E. coli Ec-CDT. Likewise, EGA is also shown to inhibit low-pH-dependent viral infectivity by Armstrong strain of LCMV, A/WSN/33 strain of influenza virus, vesicular stomatitis virus VSV-pseudotyped lentivirus & VSV-pseudotyped MLV, but not low-pH-independent infection of Nipah virus NiV or MLV. Although the exact molecular target remains unknown, EGA does not act as a pH neutralizing agent, nor does it inhibit endosomal recycling or trafficking to phagocytic compartments.
      FormOff-white powder
      Intert gas (Yes/No) Packaged under inert gas
      CAS number415687-81-9
      Chemical formulaC₁₆H₁₆BrN₃O
      Structure formulaStructure formula
      Purity≥93% by HPLC
      SolubilityDMSO (5 mg/ml). Use only fresh DMSO for reconstitution.
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesGillespie, E.J., et al. 2013. Proc. Natl. Acad. Sci. USA 110, E4904.