Millipore Sigma Vibrant Logo

HIGFBMAG-53K MILLIPLEX® Human IGF Binding Protein Magnetic Bead Panel - Metabolism Multiplex Assay

The Human IGF Binding Protein Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 7 IGFBP protein biomarkers in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Metabolism Research Sub-Category: Metabolic Disorders
View Products on Sigmaaldrich.com
HIGFBMAG-53K
Design And Price Your Kit

Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IGFBP-1 IGFBP-2 IGFBP-3 IGFBP-4 IGFBP-5 IGFBP-6 IGFBP-7 HMplexLuminex xMAP
Description
Catalogue NumberHIGFBMAG-53K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human IGF Binding Protein Magnetic Bead Panel - Metabolism Multiplex Assay
OverviewThe MILLIPLEX® Human IGF Binding Protein (IGFBP) Magnetic Bead Panel, is used for the simultaneous quantification of any or all of the following analytes in human serum, plasma, and cell culture conditioned-media samples: IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, and IGFBP-7
Background InformationInsulin-like growth factor (IGF) binding proteins (IGFBP) are expressed by multiple tissues and cells, including hepatic cells. By binding both IGF-1 and IGF-2, IGFBPs play a critical role in the regulation of IGF action, altering IGF interaction with cell surface receptors and increasing circulating IGF half-life and bioavailability. While their functions at the cellular level are not fully understood, studies have demonstrated the capacity to both stimulate and inhibit IGF-1 activity, depending on the system involved and the IGFBP studied. The ability of IGFBPs to regulate cell sensitivity by inhibiting IGF signaling may function in the control of tumor cell growth.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • IGFBP-1: Intra-assay <10% C Inter-assay <15%
  • IGFBP-2: Intra-assay <10% CV Inter-assay <15%
  • IGFBP-3: Intra-assay <10% CV Inter-assay <15%
  • IGFBP-4: Intra-assay <10% CV Inter-assay <15%
  • IGFBP-5: Intra-assay <10% CV Inter-assay <15%
  • IGFBP-6: Intra-assay <10% CV Inter-assay <15%
  • IGFBP-7: Intra-assay <10% CV Inter-assay <15%
Panel TypeMAGNETIC Endocrine
Quality LevelMQ200
Applications
ApplicationThe Human IGF Binding Protein Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 7 IGFBP protein biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• To prevent possible IGFBP proteolysis, it is recommended that a protease inhibitor cocktail solution be added to each blood sample after blood collection.
• A 2-hour sample incubation may be used, however, for optimal sensitivity, use an overnight (16-18 hour) incubation.
• Serum and plasma samples require (recommend) a 1:25 dilution with Assay Buffer.
• Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Species Reactivity
  • Human
Analytes Available
  • IGFBP-1
  • IGFBP-2
  • IGFBP-3
  • IGFBP-4
  • IGFBP-5
  • IGFBP-6
  • IGFBP-7
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • IGFBP-1 102%
  • IGFBP-2 90%
  • IGFBP-3 95%
  • IGFBP-4 97%
  • IGFBP-5 79%
  • IGFBP-6 105%
  • IGFBP-7 98%
Physicochemical Information
Sensitivity
  • MinDC+2SD
  • IGFBP-1: 0.01 ng/mL
  • IGFBP-2: 1.09 ng/mL
  • IGFBP-3: 0.15 ng/mL
  • IGFBP-4: 0.96 ng/mL
  • IGFBP-5: 9.87 ng/mL
  • IGFBP-6: 0.12 ng/mL
  • IGFBP-7: 0.07 ng/mL
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • IGFBP-1: 0.03 - 25 ng/mL
  • IGFBP-2: 0.34 - 250 ng/mL
  • IGFBP-3: 0.07 - 50 ng/mL
  • IGFBP-4: 0.69 - 500 ng/mL
  • IGFBP-5: 1.37 - 1000 ng/mL
  • IGFBP-6: 0.14 - 100 ng/mL
  • IGFBP-7: 0.07 - 50 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HIGFBMAG-53K 04053252987373

Documentation

Protocols

Title
Protocol- HIGFBMAG-53K

MILLIPLEX® Human IGF Binding Protein Magnetic Bead Panel - Metabolism Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms: Beyond fit for purpose
Custom Immunoassay Menu of Services
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HIGFBMAG-53K xPonent Protocol Template Version Magpix
Quality Control Range Sheet- IGFBP-113 and IGFBP-213

Data Sheet

Title
Full Service Custom-built Assays