Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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48-602MAG
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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HCY4MG-64K-PX20
MilliporeMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel IV, Premixed 20 Plex Assay
The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Premix Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 20 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.
More>>The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Premix Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 20 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples. Less<<
MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel IV, Premixed 20 Plex Assay
Overview
EMD Millipore’s MILLIPLEX® MAP Cytokine/Chemokine Magnetic Bead Panel IV Premix is a 20-plex premixed kit to be used for the simultaneous quantification of all of the following analytes in serum, plasma or culture supernatant samples: BAFF/Blys, BRAK/CSCL14, CCL28, CXCL16, HMGB1, IFNβ, IL-14, IL-19, IL-24, IL-28B, IL-32α, IL-34, IL-35, IL-36β, IL-37, IL-38, MIP-4/PARC/CCL18, MPIF-1/CL23 and YKL40/CHI3L1.
MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.
Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel
EMD Millipore’s MILLIPLEX® MAP Human Cytokine/Chemokine Magnetic Bead Panel IV is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background Information
Inflammation and immune cell response are key to maintaining homeostasis. The more we understand mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease: certainly immunological diseases, such as allergic reactions, asthma, infectious disease and an ever-growing number of conditions under the autoimmune umbrella, but also neurodegenerative disease, metabolic and cardiovascular disease and even cancer. The ability to measure multiple inflammation biomarkers simultaneously using EMD Millipore’s MILLIPLEX ® MAP panels, based on the Luminex xMAP® technology, to establish disease profiles that can be used for screening, diagnosis and response to treatment is critical for understanding the pathogenesis of these diseases.
References
Product Information
Detection method
Luminex xMAP
Configuration
Premixed
Precision, %
Intra-assay: 10
Inter-assay:15-25
Panel Type
MAGNETIC Cytokines/Chemokines
Applications
Application
The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Premix Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 20 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.
Key Applications
Multiplexing
Application Notes
• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• A maximum of 25 μL per well of 1:4 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay. Please note that HMGB1 can be measured in serum and plasma samples only.
Biological Information
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
Human
Analytes Available
BAFF/Blys
BRAK/CXCL14
CCL28
CXCL16
HCC-4/CCL16
HMGB1
IFNβ
IL-14
IL-19
IL-24
IL-28B
IL-36β/IL-1F8
IL-32α
IL-34
IL-35
IL-37/IL-1F7
IL-38/IL-1F10
MIP-4
MPIF-1/CCL23
YKL40
Cross Reactivity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
BAFF/Blys: 101%
BRAK/CSCL14: 101%
CCL28: 91%
CXCL16: 103%
HCC-4/CCL16: 110%
HMGB1: 104%
IFNβ: 97%
IL-14: 104%
IL-19: 106%
IL-24: 99%
IL-28B: 101%
IL-32α: 89%
IL-34: 110%
IL-35: 95%
IL-36β: 98%
IL-37: 99%
IL-38: 104%
MIP-4/PARC/CCL18: 101%
MPIF-1/CL23: 102%
YKL40/CHI3L1: 100%
Physicochemical Information
Sensitivity
MPIF-1: 7.8 pg/mL
BRAK: 607 pg/mL
CXCL16: 4.7 pg/mL
HCC-4: 28.4 pg/mL
MIP-4/PARC: 6.6 pg/mL
IL-34: 121.5 pg/mL
IL-24: 0.8 ng/mL
IL-35: 0.8 ng/mL
IL-37/IL-1F7: 5.2 pg/mL
IL-19: 248.2 pg/mL
CCL28: 100.8 pg/mL
HMGB1/HMG1: 10.5 ng/mL
IFNβ: 375.6 pg/mL
IL-38: 33.2 pg/mL
IL-28B/IFN-λ3: 17.2 pg/mL
BAFF/BLyS: 0.9 ng/mL
IL-14/α-Taxilin: 27.7 pg/mL
IL-36β: 3.5pg/mL
IL-32α: 32.1 pg/mL
YKL40/CHI3L1: 0.30 ng/mL
Standard Curve Range
BAFF/Blys: 0.44-400 ng/mL
BRAK/CXCL14: 488-500,000 pg/mL
CCL28: 98-100,000 pg/mL
CXCL16: 3-3,000 pg/mL
HMGB1: 2.0-2,000 ng/mL
IFNβ: 488 – 500,000 pg/mL
IL-14: 24-25,000 pg/mL
IL-19: 244-250,000 pg/mL
IL-24: 0.7-750 ng/mL
IL-28B: 29-30,000 pg/mL
IL-32α: 39-40,000 pg/mL
IL-34: 98-100,000 pg/mL
IL-35: 0.8-800 ng/mL
IL-36β: 2- 2,000 pg/mL
IL-37: 5 – 5,000 pg/mL
IL-38: 49-50,000 pg/mL
MIP-4/PARC/CCL18: 5-5,000 pg/mL
MPIF-1/CL23: 10-10,000 pg/mL
YKL40/CHI3L1: 0.1-100 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Recommended storage for kit components is 2 - 8°C.
