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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

PRCYTOMAG-40K MilliporeMILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
References
Technical Information
Protocols
Posters

Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology

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PRCYTOMAG-40K

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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

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Key Specifications Table

Analytes Available	Species Reactivity	Key Applications	Detection Methods
G-CSF GM-CSF IFN-γ IL-1ra IL-1β IL-2 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12/23 (p40) IL-13 IL-15 IL-17 IL-18 MCP-1 MIP-1α MIP-1β sCD40L TGF-α TNF-α VEGF	NhP	Mplex	Luminex xMAP

Description
Catalogue Number	PRCYTOMAG-40K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay
Overview	To identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include: • More flexible plate and plate washer options • Improved performance with turbid serum/plasma samples • Assay results equivalent to non-magnetic beads • Automated magnetic washing avoids many problems associated with vacuum filtration washing Therefore, the MILLIPLEX MAP Non-Human Primate Cytokine / Chemokine Panel enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity.
Background Information	The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.

References

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Inter-assay: 8 - 15 Intra-assay: 2 - 5
Panel Type	Non-Magnetic Cytokines/Chemokines
Quality Level	MQ200

Applications
Application	Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended. • This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Non-human Primate
Analytes Available	G-CSF GM-CSF IFN-γ IL-1ra IL-1β IL-2 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12/23 (p40) IL-13 IL-15 IL-17 IL-18 MCP-1 MIP-1α MIP-1β sCD40L TGF-α TNF-α VEGF
Assay Panel	Everything you need in a single kit.
Cross Reactivity	The multiplexed assay panel has been tested with samples from multiple non-human primate species, please see protocol for details.
Accuracy	70% - 101%

Physicochemical Information
Sensitivity	Refer to kit protocol for sensitivities for individual cytokines/chemokines
Standard Curve Range	IL-4: 4.9 - 20,000 pg/mL IL-10, IL-18: 12.2 - 50,000 pg/mL sCD40L, TNF-α: Refer to QC analysis sheet for exact concentration G-CSF, GM-CSF, IFN-γ, IL-1ra, IL-1β, IL-2, IL-5, IL-6, IL-8, IL-12/23 (p40), IL-13, IL-15, IL-17, MCP-1, MIP-1α, MIP-1β, TGF-α, VEGF: 2.4 - 10,000 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
PRCYTOMAG-40K	04053252988004

Documentation

Protocols

Title
Protocol- PRCYTOMAG-40K

MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay SDS

Title
Safety Data Sheet (SDS)

References

Reference overview	Pub Med ID
The Lyme disease spirochete Borrelia burgdorferi induces inflammation and apoptosis in cells from dorsal root ganglia. Ramesh, G; Santana-Gould, L; Inglis, FM; England, JD; Philipp, MT Journal of neuroinflammation 10 88 2013 Show Abstract Lyme neuroborreliosis (LNB), caused by the spirochete Borrelia burgdorferi, affects both the peripheral and the central nervous systems. Radiculitis or nerve root inflammation, which can cause pain, sensory loss, and weakness, is the most common manifestation of peripheral LNB in humans. We previously reported that rhesus monkeys infected with B. burgdorferi develop radiculitis as well as inflammation in the dorsal root ganglia (DRG), with elevated levels of neuronal and satellite glial cell apoptosis in the DRG. We hypothesized that B. burgdorferi induces inflammatory mediators in glial and neuronal cells and that this inflammatory milieu precipitates glial and neuronal apoptosis.To model peripheral neuropathy in LNB we incubated normal rhesus DRG tissue explants with live B. burgdorferi ex vivo and identified immune mediators, producer cells, and verified the presence of B. burgdorferi in tissue sections by immunofluorescence staining and confocal microscopy. We also set up primary cultures of DRG cells from normal adult rhesus macaques and incubated the cultures with live B. burgdorferi. Culture supernatants were subjected to multiplex ELISA to detect immune mediators, while the cells were evaluated for apoptosis by the in situ TUNEL assay. A role for inflammation in mediating apoptosis was assessed by evaluating the above phenomena in the presence and absence of various concentrations of the anti-inflammatory drug dexamethasone. As Schwann cells ensheath the dorsal roots of the DRG, we evaluated the potential of live B. burgdorferi to induce inflammatory mediators in human Schwann cell (HSC) cultures.Rhesus DRG tissue explants exposed to live B. burgdorferi showed localization of CCL2 and IL-6 in sensory neurons, satellite glial cells and Schwann cells while IL-8 was seen in satellite glial cells and Schwann cells. Live B. burgdorferi induced elevated levels of IL-6, IL-8 and CCL2 in HSC and DRG cultures and apoptosis of sensory neurons. Dexamethasone reduced the levels of immune mediators and neuronal apoptosis in a dose dependent manner.In this model, B. burgdorferi induced an inflammatory response and neuronal apoptosis of DRG. These pathophysiological processes could contribute to peripheral neuropathy in LNB.	23866773

Reference overview

Pub Med ID

The Lyme disease spirochete Borrelia burgdorferi induces inflammation and apoptosis in cells from dorsal root ganglia.
Ramesh, G; Santana-Gould, L; Inglis, FM; England, JD; Philipp, MT
Journal of neuroinflammation 10 88 2013

Show Abstract

Lyme neuroborreliosis (LNB), caused by the spirochete Borrelia burgdorferi, affects both the peripheral and the central nervous systems. Radiculitis or nerve root inflammation, which can cause pain, sensory loss, and weakness, is the most common manifestation of peripheral LNB in humans. We previously reported that rhesus monkeys infected with B. burgdorferi develop radiculitis as well as inflammation in the dorsal root ganglia (DRG), with elevated levels of neuronal and satellite glial cell apoptosis in the DRG. We hypothesized that B. burgdorferi induces inflammatory mediators in glial and neuronal cells and that this inflammatory milieu precipitates glial and neuronal apoptosis.To model peripheral neuropathy in LNB we incubated normal rhesus DRG tissue explants with live B. burgdorferi ex vivo and identified immune mediators, producer cells, and verified the presence of B. burgdorferi in tissue sections by immunofluorescence staining and confocal microscopy. We also set up primary cultures of DRG cells from normal adult rhesus macaques and incubated the cultures with live B. burgdorferi. Culture supernatants were subjected to multiplex ELISA to detect immune mediators, while the cells were evaluated for apoptosis by the in situ TUNEL assay. A role for inflammation in mediating apoptosis was assessed by evaluating the above phenomena in the presence and absence of various concentrations of the anti-inflammatory drug dexamethasone. As Schwann cells ensheath the dorsal roots of the DRG, we evaluated the potential of live B. burgdorferi to induce inflammatory mediators in human Schwann cell (HSC) cultures.Rhesus DRG tissue explants exposed to live B. burgdorferi showed localization of CCL2 and IL-6 in sensory neurons, satellite glial cells and Schwann cells while IL-8 was seen in satellite glial cells and Schwann cells. Live B. burgdorferi induced elevated levels of IL-6, IL-8 and CCL2 in HSC and DRG cultures and apoptosis of sensory neurons. Dexamethasone reduced the levels of immune mediators and neuronal apoptosis in a dose dependent manner.In this model, B. burgdorferi induced an inflammatory response and neuronal apoptosis of DRG. These pathophysiological processes could contribute to peripheral neuropathy in LNB.

23866773

Technical Info

Title
96 well Template 2 Up
96 well Template Single
PRCYTOMAG-40K xPonent Protocol Template Version 3.1
PRCYTOMAG-40K xPonent Protocol Template Version Magpix
Quality Control Range Sheet- PCC-121 and PCC-221
Quality Control Range Sheet- PCC-124 and PCC-224
Quality Control Ranges: MILLIPLEX® MAP Non-Human Primate Cytokine/Chemokine Kit

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Title
MILLIPLEX®MAP Cell Signaling Wall Chart

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PRCYTOMAG-40K MilliporeMILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

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Overview

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Key Specifications Table

Documentation

Protocols

MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay SDS

References

Technical Info

Posters

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