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MMYOMAG-74K MILLIPLEX® Mouse Myokine Magnetic Bead Panel

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple myokine and cytokine biomarkers in mouse serum and plasma samples.

MILLIPLEX® Mouse Myokine Magnetic Bead Panel MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Metabolism, Endocrine Research Sub-Category: Obesity, Metabolic Disorders, Inflammation
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MMYOMAG-74K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
BDNF Erythropoietin (EPO) FGF-21 Follistatin-like Protein 1 (FSTL1) IL-6 IL-15 Irisin LIF Myostatin/GDF8 Oncostatin M (OSM) Osteocrin/Musclin Osteonectin (SPARC) MMplexLuminex xMAP
Description
Catalogue NumberMMYOMAG-74K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Mouse Myokine Magnetic Bead Panel
OverviewMILLIPLEX® Mouse Myokine Magnetic Bead Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples:
• Brain-derived Neurotrophic Factor (BDNF)
• Erythropoeitin (EPO)*
• Fibroblast Growth Factor 21 (FGF21)
• Follistatin-Like Protein 1 (FSTL-1)
• IL-6
• IL-15
• Irisin
• Leukemia Inhibitory Factor (LIF)
• Myostatin (MSTN / GDF8)
• Oncostatin M (OSM)
• Osteocrin/Musclin (OSTN)
• Osteonectin (SPARC)

*Only available for cells culture samples.

MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel


EMD Millipore’s MILLIPLEX® Mouse Myokine Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationSkeletal muscle is actively involved in the synthesis and secretion of many proteins collectively termed “myokines.”

Muscle contraction during physical activity is an important activator for the release of myokines, which act in an autocrine and/or paracrine manner to regulate skeletal muscle growth. Myokines also act as endocrine hormones which mediate inter-organ crosstalk. Myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue as well as for profound changes in immune and neuron systems.

Various genetically engineered mouse strains and disease models are now serving as exceptional research tools for human myokine study.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: <10%
  • Inter-assay: <15%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationInflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple myokine and cytokine biomarkers in mouse serum and plasma samples.
Key Applications
  • Multiplexing
Application Notes• This assay uses 25 µL of a 1:1 dilution of serum or plasma per well, or 25 µl neat or appropriately diluted tissue culture supernatant per well.
• For optimal sensitivity, use an overnight (16-18 hour) incubation. A two hour incubation can also be used (though sensitivity may be lower).
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • BDNF
  • Erythropoietin (EPO)
  • FGF-21
  • Follistatin-like Protein 1 (FSTL1)
  • IL-6
  • IL-15
  • Irisin
  • LIF
  • Myostatin/GDF8
  • Oncostatin M (OSM)
  • Osteocrin/Musclin
  • Osteonectin (SPARC)
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • BDNF: 0.6-9,000 pg/mL
  • EPO: 22- 350,000 pg/mL
  • FGF21: 5- 75,000 pg/mL
  • FSTL1: 160- 2,500,000 pg/mL
  • IL-6: 9- 140,000 pg/mL
  • IL-15: 48- 750,000 pg/mL
  • Irisin: 26- 400,000 pg/mL
  • LIF: 3- 40,000 pg/mL
  • Myostatin: 256- 4,000,000 pg/mL
  • Oncostatin M: 16- 250,000 pg/mL
  • Osteocrin: 3- 40,000 pg/mL
  • Osteonectin (SPARC): 128- 2,000,000 pg/mL
Physicochemical Information
Standard Curve Range
  • See protocol
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MMYOMAG-74K 04054839329630

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Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits