Millicell® μ-Angiogenesis Activation and Inhibition Assay Kits
Related Resources
Recommended Products
-
14-572 Sigma-Aldrich PLK3 Protein, active, 10 µg -
70748 Millipore Pellet Paint® NF Co-Precipitant -
1057410250 Supelco Molecular sieve 0.3 nm -
55714 Millipore Ribonucleic Acid, Yeast - CAS 63231-63-0 - Calbiochem -
1125130010 Supelco Trifluoroacetic anhydride -
MABC1120-25UG Sigma-Aldrich Anti-PD-L2 Antibody, clone 366C.9E5 -
1715-A4050N Millipore N GS, HP APPLI, 5X1000ML -
100219 Supelco Amidosulfuric acid -
1197780100 Supelco Calcium standard solution -
64-202BP Millipore Visualizer™ Western Blot Detection Kit, rabbit
Overview
Specifications
Ordering Information
Documentation
Related Products & Applications
Related Products By: Brand Facete
| Millicell® |
Categories
| Life Science Research > Cell Analysis > Cell-based Assays > Angiogenesis & Endothelial Transmigration Assays |
Studying how compounds affect angiogenesis, either to promote or inhibit new capillary tube formation can lead to therapies affecting wound healing, tissue regeneration, cardiovascular disease, stroke, tumor progression, and more. The Millicell μ-Angiogenesis activation and inhibition kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution.
Benefits
- Continuous, live monitoring of cells as tubules form
- Even, flat slide surface prevents any liquid meniscus from forming, eliminating out-of-focus areas
- Full well visualization at low magnification
- Optimized assay conditions in a miniaturized format
- Compatible with multi-channel pipettes and fixation reagents
- Less reagents and cells needed, reducing costs and waste by up to 80% • All-in-one slide used to grow, assay, and stain cells
Supporting Data
μ-Angiogenesis Inhibition Kit
Increasing concentrations of sulforaphane resulted in both mean tube length and mean number of branch points as shown below in bright field and calcein-AM micrographs of HUVEC cells.
)
Increasing concentrations of sulforaphane resulted in both mean tube length and mean number of branch points as shown below in bright field and calcein-AM micrographs of HUVEC cells.
μ-Angiogenesis Activation Kit
(Left to right) Bright field, calcein-AM, DAPI, and calcein-DAPI merge micrographs show robust tubule formation and lumen structure in the presence of phorbol myristate acetate (PMA, a pro-angiogenic agent). Images shown are of HUVEC cells.
)
With no stimulant
)
With 50ng/mL PMA and 1x ITS
)
Lumen structure
Applications
Angiogenesis Assays