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475941 Myokinase, Yeast

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475941
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475941-1KU
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      Description
      OverviewNative, yeast myokinase. Myokinase reversibly transfers phosphate from ATP to AMP, thus forming two molecules of ADP. The enzyme is specific for adenine nucleotides and catalyzes the reaction only in the presence of a divalent metal ion (e.g. Mg2+, Ca2+, Co2+, Mn2+, or Ni2+).
      Note: 1 KU = 1000 units.
      Catalogue Number475941
      Brand Family Calbiochem®
      SynonymsAdenylate Kinase, ATP:AMP Phosphotransferase
      References
      ReferencesMakarchikov, A.F., et al. 2002. Biochem. Biophys. Acta. 1592, 117.
      Schricker, R., et al. 2002. J. Biol. Chem. 277, 28757.
      Magdolen, V., et al. 1987. Curr. Genet. 12, 405.
      Tomasselli, A.G., et al. 1986. Eur. J. Biochem. 155, 111.
      Ito, Y., et al. 1980. Eur. J. Biochem. 105, 85.
      Product Information
      CAS number9013-02-9
      Activity≥10 units/mg solid
      Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1.0 µmol ATP and 1.0 µmol AMP to 2 µmol ADP per min at 25°C, pH 7.5. Note: 1 KU = 1000 units.
      EC number2.7.4.3
      FormLyophilized
      FormulationLyophilized from potassium phosphate buffer.
      Hygroscopic Hygroscopic
      PI5.7
      Quality LevelMQ100
      Biological Information
      Specific Activity≥200 units/mg
      Physicochemical Information
      ContaminantsATPase: ≤0.01%; Phosphoglycerate kinase: ≤0.1%
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Global Trade Item Number
      Catalog Number GTIN
      475941-1KU 04055977184259