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SCC196 N27-A Rat Dopaminergic Neural Cell Line

N27-A rat dopaminergic neural cell line is a suitable model for Parkinson’s disease research.

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SCC196
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC196
      DescriptionN27-A Rat Dopaminergic Neural Cell Line
      Overview N27-A is a clonal derivative of N27 characterized by consistently high expression of the dopaminergic markers TH, dopamine transporter (DAT), and Tuj1 (3). N27-A cells express the dopaminergic neuron transcription factors Nurr1, En1, FoxA2, and Pitx3 as well as the monoamine transporter VMAT2, but do not express dopamine-beta-hydroxylase (DβH), the enzyme that converts dopamine to norepinephrine. N27-A cells exhibit higher sensitivity to neurotoxins as compared to parental N27 cells, and release dopamine under both basal and depolarization conditions. These features make the N27-A cell line an improved model for Parkinson’s disease research.

      Source:
      The N27-A cell line is a clonal derivative of the parental N27 cell line selected for high expression of dopaminergic neural markers. The parental cell line was originally isolated from dopaminergic neurons from an embryonic day 14 rat mesencephalon and immortalized with SV40 large T antigen (2).
      Background InformationParkinson’s disease is characterized by the death of dopaminergic neurons in the substantia nigra of the brain (1). In vitro models are critical for understanding the molecular pathology of Parkinson’s disease. The N27 immortalized rat dopaminergic cell line has been widely utilized in Parkinson’s research for over 20 years. However long-term passaging has led the N27 cell line to become a mixture of cell types with widely variable expression of dopaminergic neural markers such as tyrosine hydroxylase (TH).

      References:
      1. Dexter DT, Jenner P (2013). Free Radic Biol Med 62: 132-144.
      2. Clarkson ED, Rosa FG, Edwards-Prasad J, Weiland DA, Witta SE, Freed CR, Prasad KN (1998) Proc Natl Acad Sci USA 95(3): 1265-1270.
      3. Gao L, Zhou W, Symmes B, Freed CR (2016) PLoS One 11(8): e0160847.
      References
      Product Information
      Applications
      ApplicationN27-A rat dopaminergic neural cell line is a suitable model for Parkinson’s disease research.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Neural Lineage Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ ≥1X10⁶ cells/vial.
      • Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      SCC196 04054839736124