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14-440 PKA Protein, Recombinant, 10 µg

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

PKA Protein, Recombinant, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-440
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-440
      Replaces14-114
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPKA Protein, Recombinant, 10 µg
      OverviewRecombinant human full length PKA, catalytic subunit type alpha, untagged
      References
      Product Information
      HS Code3507 90 19
      Quality LevelMQ100
      Applications
      ApplicationRecombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed in E.coli BL21 (DE3) pLysS cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummarycAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
      Gene Symbol
      • PRKACA
      • MGC102831
      • PKACA
      • MGC48865
      Protein TargetPKA
      Purification MethodPhosphocellulose P11 chromatography
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
      SIZE: 351 amino acids; 40590 Da
      SUBUNIT: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
      TISSUE SPECIFICITY: Isoform 2 is sperm specific.
      PTM: Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity).
      SIMILARITY: SwissProt: P17612 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
      Molecular Weight40.7kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Kemptide substrate in vitro
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-440 04053252588150

      Documentation

      PKA Protein, Recombinant, 10 µg SDS

      Title

      Safety Data Sheet (SDS) 

      PKA Protein, Recombinant, 10 µg Certificates of Analysis

      TitleLot Number
      PKA, catalytic subunit, recombinant - D8MN079U-I D8MN079U-I
      PKA, catalytic subunit, recombinant - D8MN079U-J D8MN079U-J
      PKA, catalytic subunit, recombinant - D8MN079U-K D8MN079U-K
      PKA, catalytic subunit, recombinant - D8MN079U-M D8MN079U-M

      References

      Reference overviewApplicationPub Med ID
      Cell surface expression of the ROMK (Kir 1.1) channel is regulated by the aldosterone-induced kinase, SGK-1, and protein kinase A
      Yoo, D., et al
      J Biol Chem, 278:23066-75 (2003)  2003

      		Kinase Assay12684516 12684516
      Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase.
      Yonemoto, W M, et al.
      Meth. Enzymol., 200: 581-96 (1991)  1991

      		1956337 1956337

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-440M PKA Protein, Recombinant, 250 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases