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539578 PKG, Iα, Bovine Lung

PKG, Iα, Bovine Lung MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: cGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα

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539578
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Overview

Replacement Information

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539578-4UG
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      		 Plastic ampoule 4 μg
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      Description
      OverviewNative PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.
      Note: 4µg = 6000 units.
      Catalogue Number539578
      Brand Family Calbiochem®
      SynonymscGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα
      Application Data
      References
      ReferencesBrophy, M.C., et al. 2006. J. Vasc. Res. 39, 95.
      Colbran J.L., et al. 1992. J. Biol. Chem. 267, 9589.
      Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem 258, 11391.
      Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem 66, 253.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.
      FormLiquid
      FormulationIn 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥1300 units/µg enzyme
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      539578-4UG 04055977194746

      Documentation

      PKG, Iα, Bovine Lung SDS

      Title

      Safety Data Sheet (SDS) 

      PKG, Iα, Bovine Lung Certificates of Analysis

      TitleLot Number
      539578

      References

      Reference overview
      Brophy, M.C., et al. 2006. J. Vasc. Res. 39, 95.
      Colbran J.L., et al. 1992. J. Biol. Chem. 267, 9589.
      Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem 258, 11391.
      Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem 66, 253.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-September-2007 RFH
      SynonymscGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα
      Application Data
      DescriptionNative PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.
      FormLiquid
      FormulationIn 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.
      Concentration Label Please refer to vial label for lot-specific concentration
      Specific activity≥1300 units/µg enzyme
      Unit definitionOne unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBrophy, M.C., et al. 2006. J. Vasc. Res. 39, 95.
      Colbran J.L., et al. 1992. J. Biol. Chem. 267, 9589.
      Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem 258, 11391.
      Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem 66, 253.