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DR1002 Sigma-AldrichPhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)

This PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) is validated for use in Immunoblotting, Immunocytochemistry for the detection of ATM (pSer¹⁹⁸¹).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Synonyms: Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)

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Overview

Replacement Information

Key Specifications Table

Species Reactivity	Host	Antibody Type
H, M	M	Monoclonal Antibody

Pricing & Availability

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Description
Overview	This product has been discontinued. Recognizes the ~370 kDa ATM protein phosphorylated on Ser¹⁹⁸¹ in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992.
Catalogue Number	DR1002
Brand Family	Calbiochem®
Synonyms	Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)
Application Data	Detection of human ATM phosphorylated at Ser¹⁹⁸¹ by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence. Detection of human ATM phosphorylated at (Ser¹⁹⁸¹) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence.

References
References	Horejsi, Z., et al. 2004. Oncogene 23, 3122. Abraham, R.T. 2003. Genes Dev. 15, 2177. Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499. Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.

Product Information
Declaration	Sold under license of U.S. Patent 7,108,992.
Form	Liquid
Formulation	In PBS.
Positive control	HeLa cells treated with camptothecin (Cat. No. 208925)
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry
Application Notes	Immunoblotting (0.5 µg/ml) Immunocytochemistry (1.5 µg/ml)
Application Comments	Does not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM
Immunogen	Human
Clone	10H11.E12
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Irritant
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
DR1002-50UG	04055977225891

Documentation

PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) SDS

Title
Safety Data Sheet (SDS)

PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) Certificates of Analysis

Title	Lot Number
DR1002	Enter Lot Number

References

Reference overview
Horejsi, Z., et al. 2004. Oncogene 23, 3122. Abraham, R.T. 2003. Genes Dev. 15, 2177. Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499. Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	22-August-2007 RFH
Synonyms	Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)
Application	Immunoblotting (0.5 µg/ml) Immunocytochemistry (1.5 µg/ml)
Application Data	Detection of human ATM phosphorylated at Ser¹⁹⁸¹ by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence. Detection of human ATM phosphorylated at (Ser¹⁹⁸¹) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence.
Description	Protein G purified mouse monoclonal antibody. Recognizes the ~370 kDa ATM protein phosphorylated at Ser¹⁹⁸¹.
Background	ATM (ataxia telangiectasia mutated, ACC# NP_000042) is a member of the PI-3/PI-4 kinase family and is a central mediator of the cellular response to DNA damage and cellular stress. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. ATM is a regulator of numerous downstream proteins, including the tumor suppressor proteins, p53 and BRCA1, the checkpoint kinase, CHK2, the checkpoint proteins RAD17 and RAD9, and the DNA repair protein NBS1. ATM and the functionally related ATR kinase are thought to be master regulators of cell cycle checkpoint signaling pathways that are required for the cellular response to DNA damage and as well as genome stability. Activation of ATM in response to DNA damage requires intermolecular autophosphorylation of Ser¹⁹⁸¹ and dimer dissociation.
Host	Mouse
Immunogen species	Human
Immunogen	a synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM
Clone	10H11.E12
Isotype	IgG₁
Species	human, mouse
Positive control	HeLa cells treated with camptothecin (Cat. No. 208925)
Form	Liquid
Formulation	In PBS.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Does not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Irritant
References	Horejsi, Z., et al. 2004. Oncogene 23, 3122. Abraham, R.T. 2003. Genes Dev. 15, 2177. Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499. Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.

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