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524715 Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE

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524715
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Overview

Replacement Information

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524715-50UL
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      Description
      OverviewRecombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583).
      Catalogue Number524715
      Brand Family Calbiochem®
      SynonymsPDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
      References
      ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
      Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
      Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.
      Product Information
      Activity≥50,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.
      FormLiquid
      FormulationIn sodium phosphate buffer, 20% glycerol, pH 7.2.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      524715-50UL 04055977197655

      Documentation

      Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda Certificates of Analysis

      TitleLot Number
      524715

      References

      Reference overview
      Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
      Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
      Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.

      Brochure

      Title
      Recombinant Active Phosphodiesterases Flyer
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-July-2007 JSW
      SynonymsPDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
      DescriptionRecombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 µl per lane is recommended. In immunoblotting, gives band at ~92 kDa.
      FormLiquid
      FormulationIn sodium phosphate buffer, 20% glycerol, pH 7.2.
      Activity≥50,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
      Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
      Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.