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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ProSep® Ultra Plus Chromatography Media

Designed for high throughput affinity purification of monoclonal antibodies and other proteins. More

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Overview

Specifications

Ordering Information

Documentation

References

Reference overview	Application
Determining the Useful Lifetime of Chromatography Resins: Prospective Small-Scale Studies O’Leary et al.,Biopharm, September 2001, pp 10 -18 Biopharm, September 2001, pp 10 -18 2001
Pressure-flow relationships for packed beds of compressible chromatography media at laboratory and production scale Stickel J.J.; Fotopoulos A. Biotechnology Progress, 17/4 (744-751), 2001 2001	Process development studies
Determining the useful lifetime chromatography resins: Prospective small-scale studies O'Leary R.M.; Feuerhelm D.; Peers D.; Xu Y.; Blank G.S. BioPharm ( BIOPHARM ) (United States) 2001, 14/9 (10-18) 2001	Process development studies
Affinity chromatography is vital to protein research and development Curling JM,BIOPHARM-THE APPLIED TECHNOLOGIES OF BIOPHARMACEUTICAL DEVELOPMENT , 2001, V14, N10 (OCT), P51-52 BIOPHARM-THE APPLIED TECHNOLOGIES OF BIOPHARMACEUTICAL DEVELOPMENT , 2001, V14, N10 (OCT), P51-52 2001	Process development studies
Industrial purification of pharmaceutical antibodies: development, operation, and validation of chromatography processes. Fahrner RL, Knudsen HL, Basey CD, Galan W, Feuerhelm D, Vanderlaan M, Blank GS. Biotechnol Genet Eng Rev 2001;18:301-27 2001	Process development studies
Preparative Immunoaffinity Techniques Michael Price and Kami Beyzavi; Peptide Antigens, A Practical Approach, Chapter 5 Peptide Antigens, A Practical Approach, Chapter 5 1999
Performance comparison of Protein A affinity chromatography adsorbents for purifying recombinant monoclonal antibodies Robert L Fahrner, Duncan H Whitney, Martin Vanderlaan, Gregory S Blank. Biotechnol. Appl. Biochem, Vol 30 121-128, 1999 1999
Performance comparison of Protein A affinity-chromatography sorbents for purifying recombinant monoclonal antibodies Fahrner, et al.,Biotechnol. Appl. Biochem. (1999) 30, 121-128 Biotechnol. Appl. Biochem. (1999) 30, 121-128 1999
The optimal flow rate and column length for maximum production rate of Protein A affinity chromatography R.L Fahrner, H.V. Iyer, G.S. Blank Bioprocess Engineering 21 (1999) 287-292 - Springer-Verlag. 1999	Process development studies
Development, validation and application of an ultra-sensitive two-site enzyme immunoassay for human follistatin LW Evans, S Muttukrishna and NP Groome Journal of Endocrinology, 156, 275-282, 1998 1998

FAQ

Question	Answer
What is the difference between ProSep-vA Ultra and ProSep-vA High Capacity chromatography media?	Prosep-vA Ultra uses a 700 angstrom pore size porous glass and ProSep vA High Capacity uses a 1000 Angstrom porous glass. The Prosep-vA Ultra has a greater surface area due to the smaller pore size, which allows the immobilization of a larger amount of protein A. The increased amount of protein A results in a higher capacity resin.
How do I choose between ProSep-vA Ultra versus ProSep-vA High Capacity?	Prosep-vA Ultra has a higher binding capacity than ProSep-vA High Capacity - ≥ 56mg/ml vs ≥ 40 mg/ml. ProSep-vA High Capacity has a larger pore size and may perform better when purifying fusion proteins or antibody conjugates with molecular weights above 150 kD.
What is the shelf life of the Prosep-A family of protein A affinity media?	One Year. Shelf life studies are ongoing and will be increased as data becomes available. Please contact Technical Service for further shelf life information.
What is Prosep-vA ?	ProSep-vA is native protein A derived from Staphylococcus aureus which has been immobilized onto porous glass beads. ProSep-vA is available on glass of two diffferent porosities - ProSep-vA ultra (700 angstroms) and ProSep-vA High Capacity (1000 angstroms)
Do you provide reguatory support?	A regulatory reference document is available for ProSep-vA High Capacity and will soon be available for Prosep-vA Ultra. As the raw materials and the chemistry used in the manufacture of ProSep-vA Ultra are the same as for ProSep-vA High Capacity , the regulators will be familiar with the product. ProSep-vA High Capacity has been and is widely used in various licensed MAb products and products in clinical trails.

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Categories

Biopharmaceutical Manufacturing > Downstream Processing > Chromatography > Affinity Chromatography

C7842Designed for high throughput affinity purification of monoclonal antibodies and other proteins.

ProSep® Ultra Plus media is a protein A based affinity chromatography media designed for large-scale, cost-effective purification of high titer therapeutic antibodies.

Based on the proven technology of ProSep® media, ProSep® Ultra Plus media provides increased capacity and productivity – when compared to competing resin-based technologies. Its rigid base matrix enables predictable scale-up and greater process flexibility, enabling antibody producers to reduce the equipment footprint and purify large volumes of higher titer feedstock in less time.

Pre-packed Columns

ProSep® Ultra Plus media is available in prepacked, ready-to-use, disposable columns for research and lab development scale. The MiniChrom columns and RoboColumns® are the ideal tools for performing initial media screening, scaling and optimization studies. The easy-to-use, economical small scale columns can beused with any chromatography system.

ProSep® Ultra Plus prepacked columns are available in 1 and 5 mL MiniChrom columns and 0.2 and 0.6 mL RoboColumns®.

Benefits

High capacity
Proven technology
High throughput
Flexible, low-cost operation
Reliable scale-up

ProSep® Ultra Plus Media Characteristics
Base Matrix	Controlled Pore Glass
Particle Size	60 µm
Ligand	Recombinant native protein A
Ligand Immobilization	Multipoint
Binding Capacity – Static	Typically ≥ 67 mg/mL (hIgG)
Binding Capacity – Dynamic	Typically > 50mg/mL (10% breakthrough at 3 – 6 min residence time)
Recommended Mobile Phase Velocity	Up to 600 cm/h
pH range	1.5 – 8.5
Recommended long-term storage	2 – 8 °C, plus bacteriostat
ProSep® Ultra Plus PrePacked Columns
Components	Column - Polypropylene (PP) Bed Supports - 17 μm Polypropylene/Polyethylene (PP/PE)
Connections	10 – 32 UNF 1/16 in. fingertight, PEEK or PTFE Capillaries 1/16 in. (o.d.) with 0.5 – 0.8 mm (i.d).
Column Geometries/Volumes	ProSep® Ultra Plus 2 mL 175118822 ProSep® Ultra Plus 10 mL 175118824 ProSep® Ultra Plus 100 mL 175118827 ProSep® Ultra Plus 1 L 175118830 ProSep® Ultra Plus 5 L 175118833 ProSep® Ultra Plus 10 L 175118835 ProSep® Ultra Plus 25 L 175118834 Supplied as 50% slurry in 0.1M acetate buffer, pH 5.2, 1% benzyl alcohol. MiniChrom Column 1 mL 1.25067.0001 MiniChrom Column 5 mL 1.25076.0001 RoboColumn® 0.2mL 1.25135.0001 RoboColumn® 0.6mL 1.25143.0001 Supplied in 0.1M acetate buffer, pH 5.2, 1% benzyl alcohol.
Maximum Back Pressure	20 bar
Chemical Stability	Columns are tolerant to aqueous buffers and salt solutions, 8M urea, 6M guanidine hydrochloride, organic solvents and detergents. Do not use NAOH.
Temperature Range	4 – 30 °C
Column Dimensions	8 mm (i.d.) x 20 mm (bed length) 8 mm (i.d.) x 100 mm (bed length)
Column Bed Volume	1 mL 5 mL

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