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HTS093RTA Ready-to-Assay™ FP Prostanoid Receptor Frozen Cells

Human FP GPCR Frozen Cells for calcium flux FLIPR Assays.

Host Cell: Chem-1
HTS093RTA
1 kit  
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      Overview

      Replacement Information

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      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Key Specifications Table

      SpeciesHost CellsProtein Target
      Human Chem-1FP
      Description
      Catalogue NumberHTS093RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ FP Prostanoid Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and subsequently by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGF2alpha binds specifically to the FP receptor, which couples to Gq/11 to mobilize intracellular calcium. Binding of PGF2alpha to FP receptors in the corpus luteum is required for luteolysis and subsequent parturition in mice (Sugimoto et al., 1998). PGF2alpha also decreases intraocular pressure by an FP-dependent mechanism, and an PGF2--analog--latanoprost, is used clinically in the treatment of glaucoma (Crowston et al., 2004). FP also contributes to tachycardia induced by inflammatory stimuli (Takayama et al., 2005). Chemicon's cloned human FP-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant FP expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between FP and its ligands.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Quality LevelMQ300
      Applications
      ApplicationHuman FP GPCR Frozen Cells for calcium flux FLIPR Assays.
      Key Applications
      • Ligand Binding Assays
      Biological Information
      GPCR ClassA
      Host CellsChem-1
      Protein TargetFP
      Target Sub-FamilyProstanoid
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Sales RestrictionsThis product requires a User Agreement (Label License). Please see product datasheet for further information.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPlace cells in liquid nitrogen immediately upon receipt.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      HTS093RTA 04053252629877

      Documentation

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      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells