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GF174 Sonic HedgeHog (Shh) Protein, Human Recombinant

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: Q15465
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GF174
25 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      SourcePurity
      E. coli.Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
      Description
      Catalogue NumberGF174
      DescriptionSonic HedgeHog (Shh) Protein, Human Recombinant
      Alternate Names
      • SHH
      • HHG-1
      Background InformationThe hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoietic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
      References
      Product Information
      PresentationProduct is filtered through a 0.2 micron filter before lyophilization.
      Quality LevelMQ100
      Applications
      ApplicationThe hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene.
      Biological Information
      PurityProduct is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
      SourceE. coli.
      Cross ReactivityNone
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceBioactivity assay: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C for up to 6 months from date of receipt.
      Packaging Information
      Material Size25 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      GF174 04053252691393

      Documentation

      Sonic HedgeHog (Shh) Protein, Human Recombinant Certificates of Analysis

      TitleLot Number
      Recombinant Human -2789582 2789582
      Recombinant Human Sonic HedgeHog (Shh) - 2373512 2373512
      Recombinant Human Sonic HedgeHog (Shh) - 2382207 2382207
      Recombinant Human Sonic HedgeHog (Shh) - 2388364 2388364
      Recombinant Human -2634658 2634658
      Recombinant Human -2659614 2659614
      Recombinant Human -2669996 2669996
      Recombinant Human -2691470 2691470
      Recombinant Human -2700271 2700271
      Recombinant Human -2726078 2726078
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