UCOE Expression Technology

Ubiquitous Chromatin Opening Element (UCOE) technology gives major improvements in gene expression in stably transfected mammalian cells through effects on the structure of chromatin. UCOE technology prevent transgene silencing and give consistent, stable and high-level gene expression irrespective of the chromosomal integration site. UCOE technology expression elements are small DNA elements (isolated from around house-keeping genes, which need to be active most of the time) which create a transcriptionally active open chromatin environment around an integrated transgene, maximizing its potential to be transcribed into protein irrespective of the position of the transgene in the chromosome.

Benefits

In Reach Applications

• Rapid: Grams of protein in < 30 days
  
• Boosts: Expression levels up to 20-fold higher than conventional vectors without UCOE technology
  
• Stable: Pools of cells stable > 130 generations
  
• Advantages of transient transfections: gram quantities of protein without the high costs of transfection agent for large-scale transients
  
• Easy to use: Small DNA elements, capable of making two protein chains per plasmid, e.g., antibodies
  
• Versatile: Antibodies, EPO, GPCRs, Enzymes, Cytokines, etc., expressed
  
• Compatible: Enhances industry standard platforms, e.g., CHO cells, CMV promoters, etc.
  
• Serum-free: Protein-free media used throughout
  
• Scaleable: Up to 100 L in shake flasks and fermentors
  
• Reproducible: Stably-transfected pools can be frozen, providing working stocks for further production without the need for re-transfection
  
• Transferable: UCOE-containing vectors are also suitable for large-scale manufacture

In BioProcessing Applications

• Rapid: High-yielding cell lines can be derived in < 60 days, without amplification
  
• Higher expression: > 50% of UCOE clones have higher expression than the best non-UCOE clones
  
• Stable: Cell lines stable over > 130 generations
  
• Amplification not required: UCOE technology boost’s production without amplification, with 1 – 5 copies per cell, reducing the chance of instability caused by the high copy numbers seen with amplification
  
• More productive integration events: UCOE vectors give substantially more expressing clones than non-UCOE vectors
  
• Easy to use: Small (4 – 8kb) DNA elements, capable of making two protein chains per plasmid, e.g., antibodies
  
• Versatile: Antibodies, EPO, Receptors, Enzymes, Cytokines, etc., expressed
  
• Compatible: Enhances industry standard platforms, e.g, CHO cells, CMV promoters, etc.
  
• Serum-free: Chemically-defined, protein-free media used throughout