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MABE1036

Sigma-Aldrich

Anti-MED15 Antibody, clone 9D3.1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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antibody form

purified antibody

Quality Level

clone

9D3.1
monoclonal

purified by

using protein G

species reactivity

mouse, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

western blot: suitable

isotype

IgG1κ

UniProt accession no.

General description

GST-tagged recombinant human MED15 internal fragment.

Specificity

Expected to react with all three spliced isoforms reported by UniProt (Q96RN5).

Application

Epitope: Internal (C-terminal half).

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MED15 in 10 µg of K562 cell lysate.

Physical properties

~105 kDa observed. Target band size appears larger than the calculated molecular weights of 86.8 kDa (isoform 1), 82.6 kDa (isoform 2), and 75.9 kDa (isoform 3).

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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