western blotting
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Sample Preparation and Gel Electrophoresis | Life Science Research | MilliporeSigma
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions. -
SNAP i.d.® 2.0 System - How it Works | Life Science Research | MilliporeSigma
Learn how SNAP id 2.0 protein detection system works, which applications it is compatible with, and how it optimizes western blotting conditions for reduced workflow. -
Western Blotting Protocols | Life Science Research | MilliporeSigma
Review Western blotting protocols on various applications including rapid and standard immunodetection, peptide inhibition and membrane stripping. -
Preparing cell lysates | Life Science Research | MilliporeSigma
This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot. -
Immunodetection | Life Science Research | MilliporeSigma
The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots. -
Transfer Membranes | Life Science Research | MilliporeSigma
Our transfer membranes include PVDF membranes optimized for different protein blotting applications, blotting sandwiches, and blotting filter paper. -
Chemiluminescent Westerns | Life Science Research | MilliporeSigma
We provide a broad selection of chemiluminescent westerns including HRP (horseradish peroxide) substrates, the most sensitive in blotting and premixed for convenience. -
Western Blotting | Life Science Research | MilliporeSigma
We provide a wide portfolio of western blotting products including optimized reagents for chemiluminescent and fluorescent Western blots, and the SNAP i.d.® 2.0 system. -
Western Blotting Applications Notes | Life Science Research | MilliporeSigma
Discover updated application notes for Western blotting topics and find useful insights for antibody reuse, triple well blotting, and immunodetection applications.