Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
Water used in heating baths should be clean in order to reduce the cleaning frequency of those heating baths, and prevent problems in case of unfortunate manipulation in rotavors!
The major concern is therefore the scaling, that can really spoil the heating bath quickly and lead to cumbersome maintenance. The water used in the heating bath will preferably be free of the major scaling contributors: ions such as calcium, magnesium, and strontium that combine with carbonate to precipitate. Carbonate itself is difficult to avoid, because it is generated from the CO2 that dissolve in the water upon contact with air.
Water should not be too pure on the other hand, to avoid rusting and degradation of the heating bath. In addition, it is also beneficial to remove chlorine or other oxidizing agent present in tap water to reduce rusting of the heating bath.
Water treated by reverse osmosis is acceptable for filling heating baths. The amount of ions at the origin of the scaling is significantly reduced (calcium is eliminated at 99% by reverse osmosis), but there still enough ions (sodium, chloride, potassium, …) to avoid rusting. The water has a conductivity typically ranging from 1 to 50 µS/cm.
In water purification systems, activated carbon is usually placed upstream the reverse osmosis cartridge. This activated carbon removes chlorine from the tap feed water.
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