Protein Extraction - Yeast and Plant Cell Lysis

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Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.

Cell Signaling Kits & MAPmates™

Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.

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The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr

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Select A Sample Type	For some panels, sample type will determine which analytes can be plexed together.

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Custom Premix
Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.

If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.

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Qty	Catalog Number	Ordering Description	Qty/Pack	List Price
			96-Well Plate

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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)

Qty	Catalog Number	Ordering Description	Qty/Pack	List Price
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

Space Saver Option
Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.

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Performance comparison of YeastBuster Protein Extraction Reagent and other commercial reagents

Performance comparison of YeastBuster™ Protein Extraction Reagent (Lanes 1 & 2), another commercial reagent (Lanes 3 & 4), and the glass bead method (Lanes 5 & 6).

YeastBuster™ Protein Extraction Reagent is formulated for fast, reproducible and gentle extraction of active proteins from yeast cells. Harvest your cells and resuspend in YeastBuster™ protein extraction reagent. Incubate for 15 minutes and then remove cell debris by centrifugation. Your extract is now ready to use! YeastBuster™ reagent delivers greater yields of active proteins in crude extracts. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipitis, and Schizosaccharomyces pombestrains and with plant cells.

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PopCulture® Reagentis a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.

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