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Life Science Research
 

SmartFlare™ Publications and Related Resources

SmartFlare™ Publications:

  1. McClellan et al. (2015) mRNA Detection in living cells: A next generation cancer stem cell identification technique
  2. Lahm et al (2015) Live Fluorescent RNA-Based Detection of Pluripotency Gene Expression in Embryonic and Induced Pluripotent Stem Cells of Different Species
  3. Seftor et al (2014) Melanoma Tumor Cell Heterogeneity: A Molecular Approach to Study Subpopulations Expressing the Embryonic Morphogen Nodal

SmartFlare™ technology is based on the NanoFlare technology developed by Dr. Chad Mirkin:

  1. Mirkin et al. (2014) NanoFlares for the detection, isolation, and culture of live tumor cells from human blood
  2. Mirkin et al. (2012) Multiplexed Nanoflares: mRNA Detection in Live Cells
  3. Mirkin et al. (2007) Nano-flares: probes for transfection and mRNA detection in living cells

Related Resources:

Merck:/Freestyle/BI-Bioscience/Genomic-Analysis/SmartFlare/Live-Cell-Biomarker.jpg User Guide
Merck:/Freestyle/BI-Bioscience/Genomic-Analysis/SmartFlare/Human-Embryonic-Stem-Cell.jpg Human Embryonic Stem Cell protocols for SmartFlare probes


Fluorophore Compatibility for SmartFlare™ Probes
Cyanine 5 Cyanine 3
Excitation λ
 650nm 550nm
Emission λ
 670nm 570nm
Laser Type
(excitation λ) 
 Red (633nm) Green (532nm) or Yellow (561nm)
Incompatible Dyes
(excitation λ / emission λ)
 Alexa 647 (652nm/688nm)
Dylight 633 (638nm/658nm)
DRAQ5 (647nm/681nm)
APC (650nm/660nm) 		 PE (480nm/578nm)
TRITC (547nm/572nm)
Alexa 546 (561nm/572nm) 
"Like" Dyes
(excitation λ / emission λ)
 Alexa 647 (652nm/688nm)
Dylight 633 (638nm658nm)
DRAQ5 (647nm/681nm)
APC (650nm/660nm) 		 TRITC (547nm/572nm)
Alexa 546 (561nm/572nm)