Improved digestion of hard-to-digest proteins
Ovalbumin and ovalbumin digests were analyzed as follows: lane 1: ovalbumin, chicken egg, 43 kDa; lane 2: digest incubated 3 h using ProteoExtract® All-in-One Trypsin Digestion Kit; lane 3: digest incubated 4 h using the same kit; lane 4: digest incubated 3 h using reagent from Supplier I; lane 5: digest incubated 3 h using kit from Supplier II.
Titanium dioxide allows for selective and sensitive phosphopeptide enrichment with low background from complex mixtures.
The ProteoExtract® Glycopeptide Enrichment Kit is designed to capture high-mannose, hybrid, and complex type glycosylated peptides from complex protein mixtures.
ProteoExtract® All-In-One Trypsin Digestion Kit is optimized for the digestion of proteins by trypsin either in-gel, in-solution or in-suspension. Digested samples are suitable for analysis on mass spectroscopy, HPLC, or electrophoresis.
The ProteoExtract® Phosphopeptide TiO2 Enrichment Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by a highly selective Titanium oxide solid phase.
The ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC).
Enhanced detection allows comprehensive analysis
Ovalbumin was digested using ProteoExtract® All-in-One Trypsin Digestion Kit (panel A) or according to Supplier III kit protocol (panel B). Base peak chromatograms of nano-LC/MS analyses are shown.