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114666 Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem

Actinomycin D, Streptomyces sp., CAS 50-76-0, is an anti-neoplastic antibiotic that inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I

Primary Target: serine proteases
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #Empirical Formula
      50-76-0C₆₂H₈₆N₁₂O₁₆
      Description
      OverviewAnti-neoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. At higher concentrations, DNA polymerase is inhibited. Also acts as a competitive inhibitor of serine proteases. Inhibits cell growth and colony formation in synchronized HeLa cells. Induces apoptosis in many cell lines. However, actinomycin D has also been shown to suppress etoposide-induced apoptosis in PC12 cells. Note: 1 set = 20 x 200 µg.
      Stability: heat resistant and stable between pH 2 and 11. Decomposed by strong acids and bases.
      Catalogue Number114666
      Brand Family Calbiochem®
      SynonymsDactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
      References
      ReferencesNakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
      Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
      Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
      Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
      Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
      Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
      White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
      Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
      Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
      Product Information
      CAS number50-76-0
      FormRed crystalline solid
      Hill FormulaC₆₂H₈₆N₁₂O₁₆
      Chemical formulaC₆₂H₈₆N₁₂O₁₆
      Hygroscopic Hygroscopic
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      ApplicationActinomycin D, Streptomyces sp., CAS 50-76-0, is an anti-neoplastic antibiotic that inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.
      Biological Information
      Primary Targetserine proteases
      Secondary targetcell growth and colony formation in synchronized HeLa cells
      Purity≥98% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSAU1575000
      Safety Information
      R PhraseR: 28

      Very toxic if swallowed.
      S PhraseS: 28-36/37-45


      Wear suitable protective clothing and gloves.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic & Carcinogenic / Teratogenic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      114666 0

      Documentation

      Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem Certificates of Analysis

      TitleLot Number
      114666

      References

      Reference overview
      Nakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
      Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
      Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
      Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
      Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
      Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
      White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
      Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
      Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.

      Brochure

      Title
      Antibiotics Profiler

      Citations

      Title
    • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
    • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
    • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2009 JSW
      SynonymsDactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
      DescriptionAntineoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. A DNA-intercalator with growth inhibitory activity against certain leukemias and sarcomas. At higher concentrations, DNA polymerase is inhibited. Inhibits cell growth and colony formation in synchronized HeLa cells. A potent inducer of apoptosis in many cell lines; however, actinomycin D has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. Acts as a competitive inhibitor of serine protease from microorganisms. The growth of S. aureus is inhibited by 0.25-0.75 µg/ml, whereas a concentration of 100 µg/ml is required to inhibit E. coli due to permeability differences. Heat resistant and stable between pH 2 and 11; decomposed by strong acids and bases. λmax 441 nm ±2 nm. Note: 1 set = 20 vials x 200 µg.
      FormRed crystalline solid
      CAS number50-76-0
      RTECSAU1575000
      Chemical formulaC₆₂H₈₆N₁₂O₁₆
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (1 mg/ml), Methanol, or Chloroform
      Storage Protect from light
      +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Highly Toxic & Carcinogenic / Teratogenic
      Merck USA index14, 2800
      ReferencesNakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
      Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
      Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
      Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
      Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
      Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
      White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
      Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
      Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
      Citation
    • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
    • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
    • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
      		•