Amicon® Ultra-4 Centrifugal Filter Units
Provide both ultra-fast spin times and ultra-high recoveries
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Provide both ultra-fast spin times and ultra-high recoveries Less
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Overview
Specifications
Ordering Information
Documentation
Related Products & Applications
Related Products By: Application Facete
| Protein Purification |
| Dialysis and Filtration |
Related Products By: Brand Facete
| Ultracel® |
| Amicon® |
Categories
| Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units |
- High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
- High retentate recovery of >90%
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)
- Heat-sealed membrane minimizes downstream extractables
- 100% integrity tested for reliable performance
- Convenient sample monitoring with translucent housing and volume gradations
- Direct pipette sample access eliminates processing step to recover concentrate
- High concentration factors of 80–100X
Applications
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC
- Concentration of dilute or pre-purified proteins from column eluents
- Desalting, buffer exchange and protein dialysis
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Typical Spin Times to Filtrate Volume
)
Spin conditions: 4000 xg swinging bucket rotor at 25 °C, 4 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
| Membrane NMWL, kDa |
Protein Solute | Protein Retentate Volume, mL |
Protein Retentate Recovery, % |
| 3 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
0.19 | 98.7 |
| 10 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
0.20 | 95 |
| 30 | BSA, 67 kDa (1 mg/mL) |
0.20 | 95 |
| 50 | BSA, 67 kDa (1 mg/mL) |
0.08 | 94 |
| 100 | IgG, 156 kDa (1 mg/mL) |
0.34 | 91 |
| 4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min). | |||
| Volumes | |
| Maximum Initial Sample Volume, mL* | 4 mL |
| Typical Final Concentrate Volume | 50 µL |
| Centrifugal Force | |
| Materials | |
| Membrane | Ultracel regenerated cellulose |
| Device | Housing: Styrene/butadiene Tube: Polypropylene Cap and liner: Polyethylene |
| Dimensions | |
| Active Membrane Area | 3 cm² |
| Filtrate tube or vial (centrifuge tube) | |
| Diameter | 17.3 mm |
| Length (with cap and device) | 124 mm (4.88 in.) |
| Filter unit | |
| Diameter, mm | 17.2 |
| Length, mm | 73.4 |
| *Except for 23° fixed angle rotor: 3.5 mL | |