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ABC953 Anti-AATF Antibody/Rb-binding protein Che-1

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ABC953
100 µL  
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      Overview

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IP, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABC953
      DescriptionAnti-AATF Antibody/Rb-binding protein Che-1
      Alternate Names
      • Protein AATF
      • Apoptosis-antagonizing transcription factor
      • Rb-binding protein Che-1
      Background InformationAATF/Rb-binding protein Che-1, also known as Protein Apoptosis-antagonizing transcription factor (Protein AATF), Rb-binding protein Che-1, and encoded by the gene name AATF/ CHE1/ DED/ HSPC277, is a member of the AATF family. Recent studies have indicated that AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binds to the pocket region of RB1, and consequently it may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. In addition, AATF antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. AATF/Rb-binding protein Che-1 is expressed at high levels in brain, heart, kidney, placenta and thymus.
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-AATF antibody is validated for use in WB, IC, IP, ChIP for the detection of AATF.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in U2OS, T98G, LoVo, HT29, MCF7, HeLa, LIPARI, Jurkat, and U2OS cells were immunoprecipitated with anti-Rb (Fanciulli, M., et al. (2000). FASEB. 14:904-912).
      Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
      Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in NIH 3T3 transfected cells, Synchronized NIH 3T3, HFF human fibroblasts, and HEK293 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
      Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, AT fibroblasts, NIH 3T3 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
      Immunocytochemistry Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in Synchronized NIH 3T3, HFF human fibroblasts, HEK293, Rb transfected HEK293 cells, and Che-1 transfected NIH3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
      Biological Information
      ImmunogenLinear peptide corresponding to the POLR2J domain of human AATF/Rb-binding protein Che-1.
      EpitopePOLR2J domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AATF
      • CHE1
      • DED
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~70 kDa observed. The calculated molecular weight is 63 kDa, however AATF/Rb-binding protein Che-1 has been shown as a ~70 kDa band in western blots (Guo, G., et al. (2004). J. Biol. Chem. 279:4596-4603).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected AATF/Rb-binding protein Che-1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalog Number GTIN
      ABC953 04055977122305