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ABE2597-25UG Anti-AF9 (MLLT3)

Anti-AF9 (MLLT3), Cat. No. ABE2597, is a rabbit polyclonal antibody that detects AF-9 protein and is tested for use in ChIP-seq, Immunocytochemistry, and Western Blotting

ABE2597-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2597-25UG
      DescriptionAnti-AF9 (MLLT3)
      Alternate Names
      • Protein AF-9
      • ALL1-fused gene from chromosome 9 protein
      • Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein
      • MLLT3
      • YEATS domain-containing protein 3
      Background InformationProtein AF-9 (UniProt: P42568; also known as ALL1-fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, MLLT3, YEATS domain-containing protein 3) is encoded by the MLLT3 (also known as AF9, YEATS3) gene (Gene ID: 4300) in human. AF9 is a chromatin reader component of the super elongation complex (SEC), which is shown to be essential to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. It recognizes and binds histone H3 crotonylated at lysine 9, and with slightly lower affinity histone H3 crotonylated at lysine 18. This binding is achieved through its YEATS domain (aa 8-112). It is also reported to recognize and bind histone H3 acetylated at lysine 9, but with lower affinity than crotonylated histone H3. Mutations of MLLT3 gene have been implicated in neurodevelopmental diseases, including epilepsy and ataxia. A chromosomal aberration involving MLLT3 has also been is associated with acute leukemias.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-AF9 (MLLT3), Cat. No. ABE2597, is a rabbit polyclonal antibody that detects AF-9 protein and is tested for use in ChIP-seq, Immunocytochemistry, and Western Blotting
      Key Applications
      • ChIP-seq
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected AF9 (MLLT3) in NIH3T3 cell lysate.

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected AF9 (MLLT3) in HeLa cells.

      ChIP-seq Analysis: 20 mg (1u mg /ul) from a representative lot detected AF9 (MLLT3) in MOLM-13 cells expressing Flag-AF9 (Data courtesy of Drs. Liling Wan and Dr. C. David Allis, The Rockefeller University).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human AF9 (MLLT3) protein.
      EpitopeC-terminus
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects AF9 (MLLT3) protein. It targets an epitope within 21 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MLLT3
      • AF9
      • YEATs3
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~65 kDa observed; 63.35 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AF9 (MLLT3) in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE2597-25UG 04061841990338