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MAB1501-AF555 Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate

This Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of Actin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ACTA1, ACTA UniProt Number: P68139
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MAB1501-AF555
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue NumberMAB1501-AF555
      DescriptionAnti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate
      Alternate Names
      • Actin, alpha skeletal muscle
      • Alpha-actin-1
      • Actin
      Background InformationActins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues.
      Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ratio of beta- to gamma-actin (Lessard, JL., et al (1988). Cell Motility Cytoskeleton 10(3); 349-362).
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse antibody conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of Actin.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Actin in A431, and NIH/3T3 cells.
      The unconjugated version of this antibody (Cat. No. MAB1501) has been demonstrated to perform in WB, ELISA, ICC, IF, IHC, IH(P).
      Biological Information
      ImmunogenPurified chicken gizzard actin (Lessard, 1988).
      EpitopeEpitope is conserved in all known actins.
      CloneC4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityMAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Chicken based on 100% sequence homology. To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACTA1
      • ACTA
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight40 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Actin in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB1501-AF555 04055977182033

      Documentation

      Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Actin Antibody, clone C4, Alexa Fluor® 555 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-Actin, clone C4, Alexa Fluor® 555 Conjugate - 3231525 3231525
      Anti-Actin, clone C4, Alexa Fluor® 555 Conjugate - 3872371 3872371
      Anti-Actin, clone C4, Alexa Fluor® 555 Conjugate -2722594 2722594
      Anti-Actin, clone C4, Alexa Fluor® 555 Conjugate -Q2586382 Q2586382
      Anti-Actin, clone C4, Alexa Fluor®555 Conjugate 2977853

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies