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DP08 Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10)

This Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) is validated for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation for the detection of Adenovirus 5 E1B 55 kDa.

Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Adenovirus Infected CellsRMonoclonal Antibody
      Description
      OverviewRecognizes the ~19 kDa 2 E1B and the ~55 kDa 5 E1B adenovirus proteins in HEK293 cells. Will also immunoprecipitate associated proteins such as p53 and cyclins.
      Catalogue NumberDP08
      Brand Family Calbiochem®
      Application Data
      Detection of adenovirus 5 E1B 55 kDa protein by immunocytochemistry. Sample: HEK293 cells fixed with methanol. Primary antibody: Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) (Cat. No. DP08) (4 µg/ml). Detection: fluorescence (FITC).
      References
      ReferencesWhyte, P., et al. 1988. Nature 334, 124.
      Harlow, E., et al. 1986. J. Virol. 39, 861.
      Ziff, E. and Velcich, A. 1985. Cell 40, 705.
      Houweling, A., et al. 1980. Virology 105, 537.
      Berk, A.J., et al. 1979. Cell 17, 935.
      Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
      Shiroki, K., et al. 1979. Virology 95, 127.
      Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
      Graham, F.L., et al. 1974. Biol. 39, 637.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlHS27 cells
      Positive control293 cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunocytochemistry and Immunofluoresecence Zantema, A., et al. 1985. Virology 142, 44.
      Key Applications Immunofluorescence
      Immunoprecipitation
      Application NotesImmunocytochemistry (4 µg/ml, see application references)
      Immunofluorescence (4 µg/ml, see application references)
      Immunoprecipitation (1-2 µg/sample)
      Application CommentsThis antibody will immunoprecipitate adenovirus 2 and 5 E18 proteins and associated proteins (such as Rb and cyclins) as well as stain infected cells by immunofluorescence and immunoperoxidase. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenadenovirus 5 E1B protein
      Clone9C10
      HostRat
      IsotypeIgG2b
      Species Reactivity
      • Adenovirus Infected Cells
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      DP08 0

      Documentation

      Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) Certificates of Analysis

      TitleLot Number
      DP08

      References

      Reference overview
      Whyte, P., et al. 1988. Nature 334, 124.
      Harlow, E., et al. 1986. J. Virol. 39, 861.
      Ziff, E. and Velcich, A. 1985. Cell 40, 705.
      Houweling, A., et al. 1980. Virology 105, 537.
      Berk, A.J., et al. 1979. Cell 17, 935.
      Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
      Shiroki, K., et al. 1979. Virology 95, 127.
      Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
      Graham, F.L., et al. 1974. Biol. 39, 637.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationImmunocytochemistry (4 µg/ml, see application references)
      Immunofluorescence (4 µg/ml, see application references)
      Immunoprecipitation (1-2 µg/sample)
      Application Data
      Detection of adenovirus 5 E1B 55 kDa protein by immunocytochemistry. Sample: HEK293 cells fixed with methanol. Primary antibody: Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) (Cat. No. DP08) (4 µg/ml). Detection: fluorescence (FITC).
      DescriptionPurified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with YO rat myeloma cells. Recognizes the ~19 kDa 2 E1B and the ~55 kDa adenovirus 5 E1B proteins.
      BackgroundThe early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the leftmost 11% of the viral genome, and consists of two transcriptional units, E1A and E1B. Region E1A is sufficient for partial transformation and immortalization of primary cells, whereas the E1B function is normally required for complete transformation. In addition to their essential role in transformation, E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection, and are able to either activate or repress the transcription of specific cellular gene. E1A oncogene proteins form specific complexes with cellular proteins including the anti-oncogene retinoblastoma susceptibility gene product, p105.
      HostRat
      Immunogenadenovirus 5 E1B protein
      Clone9C10
      IsotypeIgG2b
      Speciesadenovirus infected cells
      Positive control293 cells
      Negative controlHS27 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody will immunoprecipitate adenovirus 2 and 5 E18 proteins and associated proteins (such as Rb and cyclins) as well as stain infected cells by immunofluorescence and immunoperoxidase. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesWhyte, P., et al. 1988. Nature 334, 124.
      Harlow, E., et al. 1986. J. Virol. 39, 861.
      Ziff, E. and Velcich, A. 1985. Cell 40, 705.
      Houweling, A., et al. 1980. Virology 105, 537.
      Berk, A.J., et al. 1979. Cell 17, 935.
      Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
      Shiroki, K., et al. 1979. Virology 95, 127.
      Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
      Graham, F.L., et al. 1974. Biol. 39, 637.
      Application referencesImmunocytochemistry and Immunofluoresecence Zantema, A., et al. 1985. Virology 142, 44.